Chen Ying, Miao Yonghong, Huang Liyong, Li Juxiang, Sun Haiyan, Zhao Yuanzeng, Yang Jing, Zhou Wenke
Department of Pediatrics, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, China.
BMC Complement Altern Med. 2014 Mar 5;14:86. doi: 10.1186/1472-6882-14-86.
Radix Trichosanthis (RT), the dry root tuber of Trichosanthis kirilowii Maxim (Cucurbitaceae), is a traditional Chinese medicine. Although a wide range of saponin pharmacological properties has been identified, to our knowledge, this may be the first report to investigate the crude saponins from RT. The purpose of this study was to delineate the antioxidant activity both in vitro and in vivo by using ethyl acetate (EtOAc), n-butanol, and the mixture of n-butanol and EtOAc fractions.
In vitro antioxidant activity was detected by using DPPH free radical, hydrogen peroxide scavenging, and reducing power assays. After pretreatment with different fractions saponins at 2 mg/kg/d and 3 mg/kg/d of crude drug, respectively, an established CCl4 induced acute cytotoxicity model was used to evaluate the in vivo antioxidant potential by detection of superoxide dismutase (SOD), malonaldehyde (MDA), lactate dehydrogenase (LDH), and total antioxidant capacity (T-AOC) levels.
The in vitro assay showed that the antioxidant activity of all the three fractions was promising. The reducing power of the EtOAc and the mixture of n-butanol and EtOAc extracts increased in a dose dependent manner. However, both the n-butanol and the mixture of n-butanol and EtOAc fractions in low dose exhibited in a time dependent manner with prolonged reaction time. As for hydrogen peroxide scavenging capability, the n-butanol fraction mainly demonstrated a time dependent manner, whereas EtOAc fraction showed a dose dependent manner. However, in case of in vivo assay, an increase of SOD and T-AOC and decrease of MDA and LDH levels were only observed in n-butanol (2 mg/kg/d of crude drug) extracts pretreatment group.
RT saponins in n-butanol fraction might be a potential antioxidant candidate, as CCl4-induced oxidative stress has been found to be alleviated, which may be associated with the time dependent manner of n-butanol saponins in a low dose. Further studies will be needed to investigate the active individual components in n-butanol extract, in vivo antioxidant activities and antioxidant mechanisms.
天花粉为葫芦科植物栝楼（Trichosanthis kirilowii Maxim）的干燥根，是一种传统中药。尽管已鉴定出多种皂苷的药理特性，但据我们所知，这可能是首次对天花粉粗皂苷进行研究的报告。本研究的目的是通过使用乙酸乙酯（EtOAc）、正丁醇以及正丁醇与乙酸乙酯混合馏分，在体外和体内描述其抗氧化活性。
采用DPPH自由基、过氧化氢清除和还原能力测定法检测体外抗氧化活性。分别用不同馏分皂苷以2mg/kg/d和3mg/kg/d的生药量进行预处理后，使用已建立的四氯化碳诱导急性细胞毒性模型，通过检测超氧化物歧化酶（SOD）、丙二醛（MDA）、乳酸脱氢酶（LDH）和总抗氧化能力（T-AOC）水平来评估体内抗氧化潜力。
体外试验表明，所有三种馏分均具有良好的抗氧化活性。乙酸乙酯以及正丁醇与乙酸乙酯提取物的还原能力呈剂量依赖性增加。然而，低剂量的正丁醇以及正丁醇与乙酸乙酯混合馏分随反应时间延长呈时间依赖性。至于过氧化氢清除能力，正丁醇馏分主要呈时间依赖性，而乙酸乙酯馏分呈剂量依赖性。然而，在体内试验中，仅在正丁醇（2mg/kg/d生药量）提取物预处理组中观察到SOD和T-AOC增加以及MDA和LDH水平降低。
正丁醇馏分中的天花粉皂苷可能是一种潜在的抗氧化剂，因为已发现其可减轻四氯化碳诱导的氧化应激，这可能与低剂量正丁醇皂苷的时间依赖性有关。需要进一步研究来调查正丁醇提取物中的活性单一成分、体内抗氧化活性和抗氧化机制。
Zotero 插件