参与修复因暴露于酸性矿井水而受损的大肠杆菌细胞的代谢过程。
Metabolic processes involved in repair of Escherichia coli cells damaged by exposure to acid mine water.
作者信息
Wortman A T, Bissonnette G K
机构信息
Division of Plant and Soil Sciences, West Virginia University, Morgantown 26506-6057.
出版信息
Appl Environ Microbiol. 1988 Aug;54(8):1901-6. doi: 10.1128/aem.54.8.1901-1906.1988.
Abstract
Escherichia coli was stressed by exposure to filter-sterilized acid mine water. Synthetic processes required for repair of sublethally injured survivors were studied by the addition of specific metabolic inhibitors to a resuscitation broth. Inhibitors of protein, RNA, DNA, lipid, and peptidoglycan synthesis as well as uncouplers and inhibitors of electron transport and ATPase activity were used. Acid mine water injury was severe, causing damage to the outer and cytoplasmic membranes. Repair of sublethally injured cells required protein, RNA, and lipid synthesis as well as a proton motive force.
摘要
将大肠杆菌暴露于经滤膜除菌的酸性矿井水中进行应激处理。通过向复苏肉汤中添加特定的代谢抑制剂，研究了亚致死损伤幸存者修复所需的合成过程。使用了蛋白质、RNA、DNA、脂质和肽聚糖合成的抑制剂，以及电子传递和ATP酶活性的解偶联剂和抑制剂。酸性矿井水造成的损伤很严重，对细菌外膜和细胞质膜均造成了损害。亚致死损伤细胞的修复需要蛋白质、RNA和脂质的合成以及质子动力。
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