State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University , 2699 Qianjin Street, Changchun 130012, China.
ACS Nano. 2014 Apr 22;8(4):3743-51. doi: 10.1021/nn500414u. Epub 2014 Mar 10.
Stable protein one (SP1) has been demonstrated as an appealing building block to design highly ordered architectures, despite the hybrid assembly with other nano-objects still being a challenge. Herein, we developed a strategy to construct high-ordered protein nanostructures by electrostatic self-assembly of cricoid protein nanorings and globular quantum dots (QDs). Using multielectrostatic interactions between 12mer protein nanoring SP1 and oppositely charged CdTe QDs, highly ordered nanowires with sandwich structure were achieved by hybridized self-assembly. QDs with different sizes (QD1, 3-4 nm; QD2, 5-6 nm; QD3, ∼10 nm) would induce the self-assembly protein rings into various nanowires, subsequent bundles, and irregular networks in aqueous solution. Atomic force microscopy, transmission electron microscopy, and dynamic light scattering characterizations confirmed that the size of QDs and the structural topology of the nanoring play critical functions in the formation of the superstructures. Furthermore, an ordered arrangement of QDs provides an ideal scaffold for designing the light-harvesting antenna. Most importantly, when different sized QDs (e.g., QD1 and QD3) self-assembled with SP1, an extremely efficient Förster resonance energy transfer was observed on these protein nanowires. The self-assembled protein nanostructures were demonstrated as a promising scaffold for the development of an artificial light-harvesting system.
稳定蛋白 1（SP1）已被证明是设计高度有序结构的有吸引力的构建块，尽管与其他纳米物体的混合组装仍然是一个挑战。在此，我们开发了一种通过环状蛋白纳米环和球形量子点（QD）的静电自组装来构建高阶蛋白纳米结构的策略。通过 12 聚体蛋白纳米环 SP1 与带相反电荷的 CdTe QD 之间的多静电相互作用，通过杂交自组装实现了具有三明治结构的高度有序纳米线。具有不同尺寸（QD1，3-4nm；QD2，5-6nm；QD3，∼10nm）的 QD 会诱导自组装蛋白环形成各种纳米线、随后的束和水溶液中的不规则网络。原子力显微镜、透射电子显微镜和动态光散射特性证实了 QD 的尺寸和纳米环的结构拓扑在超结构形成中起着关键作用。此外，QD 的有序排列为设计光捕获天线提供了理想的支架。最重要的是，当不同尺寸的 QD（例如 QD1 和 QD3）与 SP1 自组装时，在这些蛋白纳米线上观察到了非常有效的Förster 共振能量转移。自组装蛋白纳米结构被证明是开发人工光捕获系统的有前途的支架。
