磷酸钙颗粒会增加大鼠膝关节的摩擦力。

Calcium phosphate particulates increase friction in the rat knee joint.

作者信息

Roemhildt M L, Gardner-Morse M G, Morgan C F, Beynnon B D, Badger G J

机构信息

McClure Musculoskeletal Research Center, Department of Orthopaedics and Rehabilitation, University of Vermont, Burlington, VT 05405, USA.

出版信息

Osteoarthritis Cartilage. 2014 May;22(5):706-9. doi: 10.1016/j.joca.2014.02.938. Epub 2014 Mar 7.

DOI:10.1016/j.joca.2014.02.938

PMID:24607485

Abstract

OBJECTIVE

Basic calcium phosphate (BCP) particulates are commonly found in cartilage and synovial fluid of osteoarthritis (OA) joints with the amount of BCP correlating with knee OA severity. How cartilage mineralization affects joint degeneration has yet to be determined. The objective of this study was to determine whether BCP in the synovial fluid affects the rat knee joint coefficient of friction (COF).

METHODS

The COFs of knees from both hind limbs of four mature male rats were measured post mortem using a pendulum apparatus with an infrared tracking system. The three conditions evaluated were (1) the naïve state, (2) after the injection of 100 μL of phosphate buffered saline (PBS) (sham) and (3) after the injection of 100 μL of a 1 mg/mL BCP suspension. The decrease in the pendulum amplitude (decay) was fit using two friction models: (1) a one parameter Stanton linear decay model and (2) a two parameters combination Stanton linear decay and viscous damping exponential decay model.

RESULTS

The COF increased 17.6% after injection of BCP compared to the naïve (P = 0.0012) and 16.0% compared to the saline injected (P = 0.0018) joints as derived from the one parameter model. The COF did not differ between naïve and saline injected joints. Results from the two parameters model showed a similar increase in COF after injection of BCP while the viscous damping was not significantly different between conditions.

CONCLUSIONS

The increased joint friction with BCP particulates suggests BCPs may play a role in articular surface degradation and OA development.

摘要

目的

碱性磷酸钙（BCP）颗粒常见于骨关节炎（OA）关节的软骨和滑液中，BCP的含量与膝关节OA严重程度相关。软骨矿化如何影响关节退变尚待确定。本研究的目的是确定滑液中的BCP是否会影响大鼠膝关节的摩擦系数（COF）。

方法

使用带有红外跟踪系统的摆锤装置在四只成熟雄性大鼠的双后肢膝关节死后测量COF。评估的三种情况为：（1）初始状态；（2）注射100μL磷酸盐缓冲盐水（PBS）（假手术）后；（3）注射100μL 1mg/mL BCP悬浮液后。使用两种摩擦模型拟合摆锤振幅的减小（衰减）：（1）单参数斯坦顿线性衰减模型；（2）双参数组合的斯坦顿线性衰减和粘性阻尼指数衰减模型。