Actis G C, Maran E, Bonino F, Saracco G, Rosina F, Brunetto M R, Baldi M, Rizzetto M, Verme G, Rocca B
Division of Gastroenterology, Hospital Biostatistics Center, Ospedale Molinette, Turin, Italy.
J Biol Regul Homeost Agents. 1987 Jan-Mar;1(1):45-50.
In an effort to define immunobiological parameters identifying "responders" vs "non-responders" to IFN among hepatitis patients, 16 patients with chronic active hepatitis were screened for changes of Natural Killer cell activity (NK). 10/16 patients replicated the hepatitis B virus (HBV-DNA positive) whereas 6/16 replicated the defective B virus associated delta virus (HDV-RNA positive). Patients received 9 MU/3x/weekly/3 months of recombinant IFN alpha A. Mean NK activity of the HBV-DNA patients rose significantly from 29.9 +/- 5.3 to 45 +/- 4.7 during therapy, whereas the 6/16 HDV-RNA positive patients did not show any significant increase of NK activity. Interestingly, individual HDV-RNA positive patients exhibiting boosted NK activity also showed improvement of disease confirmed by clearance of intrahepatic delta antigen at one year. No such a correlation was found amongst the HBV-DNA positive patients. These data indicate that in spite of widespread individual variability, IFN-mediated NK boost may herald delta clearance and help in identifying "responders" and "non-responders" in IFN trials.
为了确定能够区分肝炎患者中对干扰素产生“应答者”和“无应答者”的免疫生物学参数，对16例慢性活动性肝炎患者的自然杀伤细胞活性（NK）变化进行了筛查。16例患者中，10例乙肝病毒复制（HBV - DNA阳性），而6例复制缺陷型丁型肝炎病毒（HDV - RNA阳性）。患者接受了9百万单位/每周3次/共3个月的重组干扰素αA治疗。治疗期间，HBV - DNA患者的平均NK活性从29.9±5.3显著升至45±4.7，而16例HDV - RNA阳性患者中，NK活性未出现任何显著升高。有趣的是，个别NK活性增强的HDV - RNA阳性患者在1年后肝内丁型抗原清除，疾病也得到改善。在HBV - DNA阳性患者中未发现这种相关性。这些数据表明，尽管存在广泛的个体差异，但干扰素介导的NK活性增强可能预示着丁型肝炎病毒清除，并有助于在干扰素试验中识别“应答者”和“无应答者”。
