体液中溶血磷脂酸的定量方法：综述

Methods for quantifying lysophosphatidic acid in body fluids: a review.

作者信息

Jesionowska Anna, Cecerska Elzbieta, Dolegowska Barbara

机构信息

Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, 70-111 Szczecin, Poland.

出版信息

Anal Biochem. 2014 May 15;453:38-43. doi: 10.1016/j.ab.2014.02.021. Epub 2014 Mar 5.

DOI:10.1016/j.ab.2014.02.021

PMID:24613261

Abstract

Lysophosphatidic acid (LPA) is a bioactive lipid involved in cellular signal transduction. LPA plays a role in both physiological and pathological processes. Elevated levels of LPA are observed in the plasma of patients with epithelial ovarian cancer, indicating its potential as a diagnostic marker. Quantification of total LPA can be performed by radioenzymatic, fluorometric, colorimetric, or immunoezymatic assay. Determination of individual LPA molecular species requires the use of capillary electrophoresis, gas chromatography, thin layer chromatography, liquid chromatography, or a matrix-assisted laser desorption/ionization time-of-flight method connected to an appropriate detection system.

摘要

溶血磷脂酸（LPA）是一种参与细胞信号转导的生物活性脂质。LPA在生理和病理过程中均发挥作用。上皮性卵巢癌患者血浆中LPA水平升高，表明其作为诊断标志物的潜力。总LPA的定量可通过放射酶法、荧光法、比色法或免疫酶法进行。单个LPA分子种类的测定需要使用毛细管电泳、气相色谱、薄层色谱、液相色谱或与适当检测系统相连的基质辅助激光解吸/电离飞行时间法。