Danielsson Karin, Coates Philip J, Ebrahimi Majid, Nylander Elisabet, Wahlin Ylva Britt, Nylander Karin
Department of Medical Biosciences, Umeå University, SE-90185 Umeå, Sweden.
Acta Derm Venereol. 2014 Sep;94(5):526-30. doi: 10.2340/00015555-1803.
Lichen planus (LP) is a chronic mucocutaneous disease with unknown cause. Patients with LP often have both oral and genital lesions, but these conditions are often considered as separate diseases and treated accordingly. To find out which genes are differently expressed in mucosal LP compared to normal mucosa and establish whether oral and genital LP are in fact the same disease, whole genome expression analysis was performed on epithelium from 13 patients diagnosed with oral and/or genital LP and normal controls. For confirmation of keratin 4 and corneodesmosin expression, quantitative reverse-transcription PCR and immunohistochemistry were used. Many genes involved in epithelial development and differentiation are differently expressed in epithelium from LP compared to normal epithelium. Several of the differentially expressed genes are common for oral and genital LP and the same biological processes are altered which supports the fact that oral and genital LP are manifestations of the same disease. The change in gene expression indicates that differentiation is altered leading to changes in the epithelial barrier.
扁平苔藓（LP）是一种病因不明的慢性黏膜皮肤疾病。LP患者常同时出现口腔和生殖器病变，但这些情况通常被视为独立的疾病并分别进行治疗。为了找出与正常黏膜相比，黏膜型LP中哪些基因表达存在差异，并确定口腔LP和生殖器LP是否实际上为同一种疾病，我们对13例诊断为口腔和/或生殖器LP的患者的上皮组织以及正常对照进行了全基因组表达分析。为了证实角蛋白4和角质桥粒芯蛋白的表达，我们使用了定量逆转录PCR和免疫组织化学方法。与正常上皮相比，许多参与上皮发育和分化的基因在LP上皮中表达存在差异。一些差异表达基因在口腔和生殖器LP中是共同的，并且相同的生物学过程发生了改变，这支持了口腔LP和生殖器LP是同一种疾病的表现这一事实。基因表达的变化表明分化发生改变，导致上皮屏障发生变化。
