Literak Ivan, Manga Ivan, Wojczulanis-Jakubas Katarzyna, Chroma Magdalena, Jamborova Ivana, Dobiasova Hana, Sedlakova Miroslava Htoutou, Cizek Alois
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic; CEITEC VFU, Veterinary and Pharmaceutical University Brno, Brno, Czech Republic.
CEITEC VFU, Veterinary and Pharmaceutical University Brno, Brno, Czech Republic; Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
Vet Microbiol. 2014 Jul 16;171(3-4):432-5. doi: 10.1016/j.vetmic.2014.02.015. Epub 2014 Feb 16.
We aimed at Escherichia coli and Enterobacter cloacae isolates resistant to cephalosporins and fluoroquinolones and Salmonella isolates in wild birds in Arctic Svalbard, Norway. Cloacal swabs of little auks (Alle alle, n=215) and samples of faeces of glaucous gulls (Larus hyperboreus, n=15) were examined. Inducible production of AmpC enzyme was detected in E. cloacae KW218 isolate. Sequence analysis of the 1146 bp PCR product of the ampC gene from this isolate revealed 99% sequence homology with the blaACT-14 and blaACT-5 AmpC beta-lactamase genes. Four, respectively six of the identified single nucleotide polymorphisms generated amino acid substitutions in the amino acid chain. As the ampC sequence polymorphism in the investigated E. cloacae strain was identified as unique, we revealed a novel variant of the ampC beta-lactamase gene blaACT-23.
我们的研究对象是挪威北极斯瓦尔巴群岛野生鸟类中对头孢菌素和氟喹诺酮耐药的大肠杆菌和阴沟肠杆菌分离株以及沙门氏菌分离株。我们检查了侏海雀（Alle alle，n = 215）的泄殖腔拭子和北极鸥（Larus hyperboreus，n = 15）的粪便样本。在阴沟肠杆菌KW218分离株中检测到AmpC酶的可诱导产生。对该分离株ampC基因1146 bp PCR产物的序列分析显示，其与blaACT-14和blaACT-5 AmpCβ-内酰胺酶基因具有99%的序列同源性。所鉴定的四个和六个单核苷酸多态性分别在氨基酸链中产生了氨基酸替换。由于在所研究的阴沟肠杆菌菌株中ampC序列多态性被鉴定为独特的，我们发现了ampCβ-内酰胺酶基因blaACT-23的一个新变体。
