Bylda Caroline, Thiele Roland, Kobold Uwe, Volmer Dietrich A
Roche Diagnostics GmbH, Penzberg, Germany.
Analyst. 2014 May 21;139(10):2265-76. doi: 10.1039/c4an00094c.
Liquid chromatography-mass spectrometry analysis of small molecules from biofluids requires sensitive and robust assays. Because of the very complex nature of many biological samples, efficient sample preparation protocols to remove unwanted components and to selectively extract the compounds of interest are an essential part of almost every bioanalytical workflow. This review describes the most common problems encountered during sample preparation, ways to optimize established sample preparation techniques and important recent developments to reduce or eliminate major interferents from biofluids.
对生物流体中的小分子进行液相色谱-质谱分析需要灵敏且稳健的检测方法。由于许多生物样品的性质非常复杂，因此采用高效的样品制备方案以去除不需要的成分并选择性地提取目标化合物，几乎是每个生物分析工作流程的重要组成部分。本综述描述了样品制备过程中遇到的最常见问题、优化现有样品制备技术的方法以及近期为减少或消除生物流体中的主要干扰物而取得的重要进展。
