Redemann Stefanie, Weber Britta, Möller Marit, Verbavatz Jean-Marc, Hyman Anthony A, Baum Daniel, Prohaska Steffen, Müller-Reichert Thomas
Experimental Center, Medical Faculty Carl Gustav Carus , Technische Universität Dresden, Fetscherstraße 74, 01307, Dresden, Germany.
Methods Mol Biol. 2014;1136:261-78. doi: 10.1007/978-1-4939-0329-0_12.
The development of automatic tools for the three-dimensional reconstruction of the microtubule cytoskeleton is crucial for large-scale analysis of mitotic spindles. Recently, we have published a method for the semiautomatic tracing of microtubules based on 3D template matching (Weber et al., J Struct Biol 178:129-138, 2012). Here, we give step-by-step instructions for the automatic tracing of microtubules emanating from centrosomes in the early mitotic Caenorhabditis elegans embryo. This approach, integrated in the visualization and data analysis software Amira, is applicable to tomographic data sets from other model systems.
开发用于微管细胞骨架三维重建的自动化工具对于有丝分裂纺锤体的大规模分析至关重要。最近，我们发表了一种基于三维模板匹配的微管半自动追踪方法（Weber等人，《结构生物学杂志》178:129 - 138，2012年）。在此，我们给出了在早期有丝分裂的秀丽隐杆线虫胚胎中自动追踪从中心体发出的微管的详细步骤说明。这种方法集成在可视化和数据分析软件Amira中，适用于来自其他模型系统的断层扫描数据集。
