Yong Yang, Ming Zhao Dong, Feng Li, Chun Zhao Wen, Hua Wu
Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, People's Republic of China.
Department of Orthopaedics, Optical Valley School District, Hubei Hospital of Traditional Chinese Medicine, People's Republic of China.
J Tissue Eng Regen Med. 2016 Oct;10(10):E537-E545. doi: 10.1002/term.1864. Epub 2014 Mar 16.
It has been reported that electromagnetic fields (EMFs) can promote the healing of non-union, osteogenesis and differentiation of the osteoblasts. However, its mechanism has not been unravelled. In this study, we detected some response induced by EMF and evaluated the importance of these signals for EMF-induced osteogenesis in bone marrow mesenchymal stem cells (MSCs). We characterized the expression of EMF-induced osteogenesis markers in MSCs, using RT-PCR and real-time PCR. Western blot was used to detect the signalling pathways. We found that EMF could promote osteogenesis in MSCs, along with the expression of several osteogenic markers. EMF-induced cyclic adenosine monophosphate (cAMP) level increase causes protein kinase A (PKA) and extracellular signal-regulated kinase (ERK)1/2 phosphorylation. Pretreating the MSCs with the mitogen-activated protein kinase (MAPK)/ERK kinase 1/2 (MEK1/2) inhibitor PD98059, or the PKA inhibitor H-89, significantly inhibited the induction of osteogenic markers, showing that EMF induction of osteogenesis was dependent on the ERK and PKA signalling pathways. Therefore, our study showed that EMF promoted MSC osteogenesis and that the EMF-induced osteogenic markers were mediated by both the PKA and MAPK signalling pathways. Copyright © 2014 John Wiley & Sons, Ltd.
据报道，电磁场（EMFs）可促进骨不连的愈合、成骨细胞的成骨作用及分化。然而，其机制尚未阐明。在本研究中，我们检测了电磁场诱导的一些反应，并评估了这些信号对骨髓间充质干细胞（MSCs）中电磁场诱导的成骨作用的重要性。我们使用逆转录聚合酶链反应（RT-PCR）和实时聚合酶链反应（real-time PCR）对骨髓间充质干细胞中电磁场诱导的成骨标志物的表达进行了表征。蛋白质印迹法用于检测信号通路。我们发现，电磁场可促进骨髓间充质干细胞的成骨作用，并伴有几种成骨标志物的表达。电磁场诱导的环磷酸腺苷（cAMP）水平升高导致蛋白激酶A（PKA）和细胞外信号调节激酶（ERK）1/2磷酸化。用丝裂原活化蛋白激酶（MAPK）/ERK激酶1/2（MEK1/2）抑制剂PD98059或PKA抑制剂H-89预处理骨髓间充质干细胞，可显著抑制成骨标志物的诱导，表明电磁场诱导的成骨作用依赖于ERK和PKA信号通路。因此，我们的研究表明，电磁场促进骨髓间充质干细胞成骨，且电磁场诱导的成骨标志物由PKA和MAPK信号通路介导。版权所有© 2014约翰威立父子有限公司。
