Porter Nicole, Lerch Athena, Jason Leonard A, Sorenson Matthew, Fletcher Mary Ann, Herrington Joshua
DePaul University.
University of Miami.
J Behav Neurosci Res. 2010 Jun 1;8(2):1-8.
Participants with CFS were grouped into viral and non-viral onset fatigue categories and assessed for differential immunological marker expression. Peripheral Blood Mononuclear Cells were assessed for differential phenotypic expression of surface adherence glycoproteins on circulating lymphocytes. The flow cytometric analysis employed fluorescent monoclonal antibody labeling. The viral in comparison to the non-viral group demonstrated significant elevations in several Th1 type subsets including: the percentage and number of CD4+ cells, the percentage and number of CD2+CD26+ cells, the percentage and number of CD2+CD4+CD26+ cells, the percentage and number of CD4+ CD26+ cells, and the percentage of Th2 naïve cells (CD4+ CD45RA+CD62L+). Of the remaining significant findings, the non viral group demonstrated significant elevations in comparison to the viral group for the following Th1 type subsets: the percentage of CD8+ cells, the percentage of T-cytotoxic suppressor cells (CD3+8+), and the percentage and number of Th1 memory cells (CD8+CD45RA-CD62L-). The viral group demonstrated a pattern of activation that differed from that of the group with a non-viral etiology, as evidenced by an elevated and out of range percentage and number of CD4+ cells, the percentage of CD2+CD26+, and the percentage of Th2 naïve cells (CD4+CD45RA+CD62L+). Both groups demonstrated reduced and out of range Natural Killer Cell Cytotoxicity and percentage of B-1 cells (CD5+CD19). In addition, both groups demonstrated an elevated and out of range percentage of CD2+CD8+CD26+, percentage of the Th1 memory subset (CD4+CD45RA-CD62L-), the percentage of Th1 memory and naïve cells (CD8+CD45RA-CD62L-, CD8+CD45RA+CD62L-), the percentage and number of Th2 memory cells (CD4+CD45RA-CD62L+), and the percentage of Th2 memory and naïve cells (CD8+CD45RA-CD62L+, CD8+CD45RA+CD62L+). These findings imply that the homeostatic mechanism responsible for the regulation of the Th1 (cell mediated) and Th2 (humoral) immune responses is disturbed in CFS. The implications of these findings are discussed.
慢性疲劳综合征（CFS）患者被分为病毒感染引发疲劳组和非病毒感染引发疲劳组，并对其免疫标志物的差异表达进行评估。对外周血单个核细胞（PBMC）进行检测，以分析循环淋巴细胞表面黏附糖蛋白的表型差异表达。流式细胞术分析采用荧光单克隆抗体标记。与非病毒感染组相比，病毒感染组的几个Th1型亚群出现显著升高，包括：CD4+细胞的百分比和数量、CD2+CD26+细胞的百分比和数量、CD2+CD4+CD26+细胞的百分比和数量、CD4+CD26+细胞的百分比和数量，以及Th2初始细胞（CD4+CD45RA+CD62L+）的百分比。在其余的显著发现中，非病毒感染组与病毒感染组相比，以下Th1型亚群出现显著升高：CD8+细胞的百分比、细胞毒性抑制性T细胞（CD3+8+）的百分比，以及Th1记忆细胞（CD8+CD45RA-CD62L-）的百分比和数量。病毒感染组表现出一种与非病毒病因组不同的激活模式，表现为CD4+细胞的百分比和数量升高且超出范围、CD2+CD26+的百分比，以及Th2初始细胞（CD4+CD45RA+CD62L+）的百分比。两组均表现出自然杀伤细胞细胞毒性降低且超出范围，以及B-1细胞（CD5+CD19）的百分比降低。此外，两组均表现出CD2+CD8+CD26+的百分比升高且超出范围、Th1记忆亚群（CD4+CD45RA-CD62L-）的百分比、Th1记忆和初始细胞（CD8+CD45RA-CD62L-、CD8+CD45RA+CD62L-）的百分比、Th2记忆细胞（CD4+CD45RA-CD62L+）的百分比和数量，以及Th2记忆和初始细胞（CD8+CD45RA-CD62L+、CD8+CD45RA+CD62L+）的百分比升高且超出范围。这些发现表明，在慢性疲劳综合征中，负责调节Th1（细胞介导）和Th2（体液）免疫反应的稳态机制受到干扰。本文讨论了这些发现的意义。
