Braciale T J, Sweetser M T, Morrison L A, Kittlesen D J, Braciale V L
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
Proc Natl Acad Sci U S A. 1989 Jan;86(1):277-81. doi: 10.1073/pnas.86.1.277.
Two distinct regions of the influenza A/JAP/305/57 hemagglutinin molecule are identifiable as sites recognized by murine class I major histocompatibility complex (MHC) (H-2d)-restricted cytolytic T lymphocytes (CTL) generated in response to immunization with infectious type A influenza virus. Each of these sites can be mimicked by a synthetic oligopeptide of approximately 20 amino acids. Data presented herein indicate that these two sites define the dominant immunogenic epitopes on the hemagglutinin recognized by H-2Kd-restricted CTL. These same sites are not efficiently recognized by hemagglutinin-specific class I MHC-restricted CTL of several unrelated MHC haplotypes. These observations show that even for a large complex glycoprotein molecule like the influenza hemagglutinin, only a limited number of class I CTL recognition sites are generated in the infected cell and that the subset of immunogenic epitopes is dependent on the MHC haplotype of the responding individual. These parameters need to be considered in the design of synthetic and recombinant vaccines.
甲型流感病毒A/JAP/305/57血凝素分子有两个不同区域，可被小鼠I类主要组织相容性复合体（MHC）（H-2d）限制的细胞毒性T淋巴细胞（CTL）识别，这些CTL是在接种感染性甲型流感病毒后产生的。这两个位点中的每一个都可以被一个约20个氨基酸的合成寡肽模拟。本文给出的数据表明，这两个位点定义了H-2Kd限制的CTL所识别的血凝素上的主要免疫原性表位。这几个不相关的MHC单倍型的血凝素特异性I类MHC限制的CTL不能有效地识别这些相同的位点。这些观察结果表明，即使对于像流感血凝素这样的大型复杂糖蛋白分子，在受感染细胞中产生的I类CTL识别位点数量也是有限的，并且免疫原性表位的子集取决于应答个体的MHC单倍型。在设计合成疫苗和重组疫苗时需要考虑这些参数。
