Zowawi Hosam M, Sartor Anna L, Balkhy Hanan H, Walsh Timothy R, Al Johani Sameera M, AlJindan Reem Y, Alfaresi Mubarak, Ibrahim Emad, Al-Jardani Amina, Al-Abri Seif, Al Salman Jameela, Dashti Ali A, Kutbi Abdullah H, Schlebusch Sanmarié, Sidjabat Hanna E, Paterson David L
The University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, Australia King Abdulaziz Medical City, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia GCC Center for Infection Control, Riyadh, Saudi Arabia
The University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, Australia.
Antimicrob Agents Chemother. 2014 Jun;58(6):3085-90. doi: 10.1128/AAC.02050-13. Epub 2014 Mar 17.
The molecular epidemiology and mechanisms of resistance of carbapenem-resistant Enterobacteriaceae (CRE) were determined in hospitals in the countries of the Gulf Cooperation Council (GCC), namely, Saudi Arabia, United Arab Emirates, Oman, Qatar, Bahrain, and Kuwait. Isolates were subjected to PCR-based detection of antibiotic-resistant genes and repetitive sequence-based PCR (rep-PCR) assessments of clonality. Sixty-two isolates which screened positive for potential carbapenemase production were assessed, and 45 were found to produce carbapenemase. The most common carbapenemases were of the OXA-48 (35 isolates) and NDM (16 isolates) types; 6 isolates were found to coproduce the OXA-48 and NDM types. No KPC-type, VIM-type, or IMP-type producers were detected. Multiple clones were detected with seven clusters of clonally related Klebsiella pneumoniae. Awareness of CRE in GCC countries has important implications for controlling the spread of CRE in the Middle East and in hospitals accommodating patients transferred from the region.
在海湾合作委员会（GCC）国家，即沙特阿拉伯、阿拉伯联合酋长国、阿曼、卡塔尔、巴林和科威特的医院中，对耐碳青霉烯类肠杆菌科细菌（CRE）的分子流行病学及耐药机制进行了研究。对分离菌株进行基于聚合酶链反应（PCR）的耐药基因检测以及基于重复序列的PCR（rep-PCR）克隆性评估。对62株筛查出可能产生碳青霉烯酶呈阳性的分离菌株进行了评估，发现其中45株产生碳青霉烯酶。最常见的碳青霉烯酶类型为OXA-48型（35株）和NDM型（16株）；发现6株同时产生OXA-48型和NDM型碳青霉烯酶。未检测到KPC型、VIM型或IMP型产生菌。检测到多个克隆，其中有七组克隆相关的肺炎克雷伯菌。海湾合作委员会国家对CRE的认识对于控制中东地区以及接收该地区转诊患者的医院中CRE的传播具有重要意义。
