Alzoghaibi Mohammed A, Pandi-Perumal Seithikurippu R, Sharif Munir M, BaHammam Ahmed S
Department of Physiology, College of Medicine, King Saud University, Riyadh, Saudi Arabia; National Plan for Science and Technology, King Saud University, Riyadh, Saudi Arabia.
Center for Healthful Behavior Change, Division of Health and Behavior, Department of Population Health, New York University Langone Medical Center, Clinical and Translational Research Institute, New York, New York, United States of America; Somnogen Canada Inc., Toronto, Ontario, Canada.
PLoS One. 2014 Mar 17;9(3):e92214. doi: 10.1371/journal.pone.0092214. eCollection 2014.
We aimed to assess the effect of Islamic intermittent fasting, during and outside of Ramadan, on plasma levels of leptin and ghrelin while controlling for several potential confounding variables. Eight healthy male volunteers with a mean age of 26.6±4.9 years reported to the sleep disorders center (SDC) at King Saud University on four occasions: 1) adaptation; 2) 4 weeks before Ramadan while performing Islamic fasting for 1 week (baseline fasting) (BLF); 3) 1 week before Ramadan (non-fasting baseline) (BL); and 4) during the second week of Ramadan while fasting. Plasma leptin and ghrelin levels were measured using enzyme-linked immunoassays at 22:00, 02:00, 04:00, 06:00, and 11:00. During BLF, there were significant reductions in plasma leptin concentrations at 22:00 and 02:00 compared with the baseline concentrations (at 22:00: 194.2±177.2 vs. 146.7±174.5; at 02:00: 203.8±189.5 vs. 168.1±178.1; p<0.05). During Ramadan, there was a significant reduction in plasma leptin levels at 22:00 (194.2±177.2 vs. 132.6±130.4, p<0.05). No significant difference in plasma ghrelin concentrations was detected during the BL, BLF, or Ramadan periods. Cosinor analyses of leptin and ghrelin plasma levels revealed no significant changes in the acrophases of the hormones during the three periods. The nocturnal reduction in plasma leptin levels during fasting may be the result of the changes in meal times during fasting.
我们旨在评估斋月期间及斋月之外的伊斯兰间歇性禁食对瘦素和胃饥饿素血浆水平的影响，同时控制几个潜在的混杂变量。八名平均年龄为26.6±4.9岁的健康男性志愿者分四次前往沙特国王大学睡眠障碍中心（SDC）：1）适应期；2）斋月前4周，同时进行1周的伊斯兰禁食（基线禁食）（BLF）；3）斋月前1周（非禁食基线）（BL）；4）斋月第二周禁食期间。在22:00、02:00、04:00、06:00和11:00使用酶联免疫分析法测量血浆瘦素和胃饥饿素水平。在BLF期间，与基线浓度相比，22:00和02:00时血浆瘦素浓度显著降低（22:00时：194.2±177.2 vs. 146.7±174.5；02:00时：203.8±189.5 vs. 168.1±178.1；p<0.05）。在斋月期间，22:00时血浆瘦素水平显著降低（194.2±177.2 vs. 132.6±130.4，p<0.05）。在BL、BLF或斋月期间，未检测到血浆胃饥饿素浓度有显著差异。对瘦素和胃饥饿素血浆水平的余弦分析显示，在这三个时期，激素的峰值相位没有显著变化。禁食期间血浆瘦素水平的夜间降低可能是禁食期间用餐时间变化的结果。
