Mohimen A, Maitra T K, Jalan K N, Mehra S
Kothari Centre of Gastroenterology, CMRI, Calcutta, India.
J Immunol Methods. 1989 Feb 8;117(1):39-44. doi: 10.1016/0022-1759(89)90116-6.
An assay specific for immune complexes containing Entamoeba histolytica antigens has been developed. Rabbit antibody specific for human gamma globulin is attached to a solid phase (nitrocellulose). During the first incubation step polyethylene glycol-precipitated immune complexes are bound to the rabbit antibody attached to the solid phase. In the second incubation step a radiolabelled rabbit antibody specific for Entamoeba histolytica combines with E. histolytica antigens in the complexes. Quantitation of the radiolabel provides a direct measurement of the level of specific immune complexes. The principle of the method has been verified using artificial soluble immune complexes prepared by mixing a pool of serum containing a high titre of anti-E. histolytica antibodies (greater than 1:2048 by hemagglutination assay) with the E. histolytica antigen. This antigen was prepared from axenically cultured NIH:200 strains of Entamoeba histolytica. Using sera from patients with clinical amoebic disease this method detected immune complexes containing E. histolytica in a high proportion of cases.
已开发出一种针对含有溶组织内阿米巴抗原的免疫复合物的检测方法。对人γ球蛋白具有特异性的兔抗体附着于固相（硝酸纤维素）上。在第一步孵育过程中，聚乙二醇沉淀的免疫复合物与附着于固相的兔抗体结合。在第二步孵育过程中，对溶组织内阿米巴具有特异性的放射性标记兔抗体与复合物中的溶组织内阿米巴抗原结合。放射性标记的定量提供了对特异性免疫复合物水平的直接测量。该方法的原理已通过使用人工可溶性免疫复合物得到验证，这些复合物是通过将含有高滴度抗溶组织内阿米巴抗体（血凝试验大于1:2048）的混合血清与溶组织内阿米巴抗原混合制备而成。该抗原由无菌培养的溶组织内阿米巴NIH:200菌株制备。使用临床阿米巴病患者的血清，该方法在很大比例的病例中检测到了含有溶组织内阿米巴的免疫复合物。
