Boon M E, Gerrits P O, Moorlag H E, Nieuwenhuis P, Kok L P
Leiden Cytology and Pathology Laboratory, The Netherlands.
Histochem J. 1988 Jun-Jul;20(6-7):313-22. doi: 10.1007/BF01002723.
Formaldehyde is the most commonly used fixative in pathology laboratories. However, due to time pressures, this fixative is often not optimally exploited. The majority of biopsies are only partly fixed when histoprocessing is started, with adverse effects. This paper reports how formaldehyde fixation is improved, by using 1.5 min of microwave irradiation of tissue previously soaked for four hours in the fixation solution. It is argued that this beneficial effect of microwave irradiation can be attributed to the acceleration of the reaction of formaldehyde to the tissue. Formation of free formaldehyde, by the dehydration of methylene glycol present in the tissue when the irradiation starts, is also enhanced. Five different formaldehyde-containing fixatives were evaluated, using five different working protocols. Spleen was taken as a suitable tissue for these tests. The technique described leads to uniform microscopical results. It is a simple method and is suitable for use in routine laboratories.
甲醛是病理实验室最常用的固定剂。然而，由于时间紧迫，这种固定剂常常未得到最佳利用。大多数活检组织在开始组织处理时仅部分固定，会产生不良影响。本文报告了如何通过对预先在固定液中浸泡4小时的组织进行1.5分钟的微波照射来改进甲醛固定。有人认为，微波照射的这种有益效果可归因于甲醛与组织反应的加速。照射开始时，组织中存在的亚甲基二醇脱水形成游离甲醛的过程也得到了增强。使用五种不同的工作方案对五种不同的含甲醛固定剂进行了评估。脾脏被用作这些测试的合适组织。所描述的技术可产生一致的显微镜结果。这是一种简单的方法，适用于常规实验室。
