Kok L P, Boon M E, Ouwerkerk-Noordam E, Gerrits P O
J Microsc. 1986 Nov;144(Pt 2):193-9. doi: 10.1111/j.1365-2818.1986.tb02799.x.
Microwaves stimulate diffusion in tissue and affect the proteins and membrane of the cell. These actions can be used to shorten the time required to fix and prepare cell blocks from sputum. The fixative containing ethyl alcohol and polyethylene glycol is particularly suitable for microwave enhanced fixation. Applying a commercially available domestic microwave oven, in which the temperature and power level can be set, cell blocks can be prepared from fresh sputum in 35 min. The microscopical results are of a superior quality because of the microwave-induced homogeneous distribution of the effects of the applied immersion fluids on the cells. The method is very successful for all specimens containing a large amount of mucous. Health hazards for laboratory personnel are nil in this cell blocking method; in contrast to when fresh sputum containing infectious agents is used for smear preparation.
微波可刺激组织中的扩散并影响细胞的蛋白质和细胞膜。这些作用可用于缩短从痰液固定和制备细胞块所需的时间。含有乙醇和聚乙二醇的固定剂特别适合微波增强固定。使用可设置温度和功率水平的市售家用微波炉，可在35分钟内从新鲜痰液制备细胞块。由于微波使所用浸泡液对细胞的作用均匀分布，显微镜检查结果质量上乘。该方法对所有含有大量黏液的标本都非常成功。在这种细胞块制备方法中，实验室人员不存在健康危害；这与使用含有传染源的新鲜痰液制备涂片时的情况形成对比。
