Wang Bingmei, Li Hanliang, Liu Ying, Lin Xin, Lin Yao, Wang Ye, Hu Xuefeng, Zhang Yanding
Fujian Key Laboratory of Developmental and Neuro Biology, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian, 350108, People's Republic of China,
J Mol Histol. 2014 Oct;45(5):487-96. doi: 10.1007/s10735-014-9572-5. Epub 2014 Mar 20.
The WNT/β-CATENIN signaling has been demonstrated to play critical roles in mouse tooth development, but little is known about the status of these molecules in human embryonic tooth. In this study, expression patterns of WNT/β-CATENIN signaling components, including WNT ligands (WNT3, WNT5A), receptors (FZD4, FZD6, LRP5), transducers (β-CATENIN), transcription factors (TCF4, LEF1) and antagonists (DKK1, SOSTDC1) were investigated in human tooth germ at the bud, cap and bell stages by in situ hybridization. All these genes exhibited similar but slightly distinct expression patterns in human tooth germ in comparison with mouse. Furthermore the mRNA expression of these genes in incisors and molars at the bell stage was also examined by real-time PCR. Our results reveal the status of active WNT/β-CATENIN signaling in the human tooth germ and suggest these components may also play an essential role in the regulation of human tooth development.
WNT/β-连环蛋白信号通路已被证明在小鼠牙齿发育中起关键作用，但对于这些分子在人类胚胎牙齿中的状况却知之甚少。在本研究中，通过原位杂交技术，对人类牙胚在蕾状期、帽状期和钟状期时WNT/β-连环蛋白信号通路的组成成分，包括WNT配体（WNT3、WNT5A）、受体（FZD4、FZD6、LRP5）、转导分子（β-连环蛋白）、转录因子（TCF4、LEF1）和拮抗剂（DKK1、SOSTDC1）的表达模式进行了研究。与小鼠相比，所有这些基因在人类牙胚中均呈现出相似但略有不同的表达模式。此外，还通过实时PCR检测了这些基因在钟状期切牙和磨牙中的mRNA表达。我们的研究结果揭示了人类牙胚中活跃的WNT/β-连环蛋白信号通路的状况，并表明这些成分可能在人类牙齿发育的调控中也起着至关重要的作用。
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