Sperotto M M, Ipsen J H, Mouritsen O G
Department of Structural Properties of Materials, Technical University of Denmark, Lyngby.
Cell Biophys. 1989 Feb;14(1):79-95. doi: 10.1007/BF02797393.
An account is given of the current status of theoretical modeling of the phase equilibria in lipid membranes with intrinsic proteins. Special attention is paid to the description of lateral phase separation, which is important for membrane function since it may lead to biologically differentiated regions. We discuss in particular the mattress-model approach by Mouritsen and Bloom, who take matching between protein and lipid hydrophobic thicknesses as a determining factor for the phase behavior. The model has been developed in the framework of phenomenological thermodynamic solution theory. The predictions of the theory are compared to a variety of experimental measurements, including those of membrane recombinants of the protein content of the reaction center and antenna protein of the bacterial photosynthetic apparatus as well as the erythrocyte band 3 protein. The physical effects of lipid-protein interactions are contrasted to those of lipid-cholesterol interactions. The concept of hydrophobic matching is then used as a basis for discussing a possible relationship between membrane thickness and physiological function.
本文介绍了含内在蛋白的脂质膜相平衡理论建模的现状。特别关注横向相分离的描述，这对膜功能很重要，因为它可能导致生物分化区域。我们特别讨论了穆里特森和布鲁姆的床垫模型方法，他们将蛋白质和脂质疏水厚度的匹配作为相行为的决定因素。该模型是在唯象热力学溶液理论的框架内发展起来的。将该理论的预测与各种实验测量结果进行了比较，包括细菌光合装置反应中心和天线蛋白以及红细胞带3蛋白的膜重组体的测量结果。对比了脂质 - 蛋白质相互作用与脂质 - 胆固醇相互作用的物理效应。然后将疏水匹配的概念作为讨论膜厚度与生理功能之间可能关系的基础。
