School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
Research and Development Division, H-Plus Eco Ltd, BVC #301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Dec;62(Pt 12):2815-2821. doi: 10.1099/ijs.0.033365-0. Epub 2012 Jan 6.
Two Gram-reaction-negative, motile bacteria, designated strains MJ01(T) and MJ14, were isolated from sludge collected from the Daejeon sewage disposal plant in South Korea. The taxonomic positions of both strains were determined using a polyphasic approach. In phylogenetic analyses based on 16S rRNA gene sequences, strains MJ01(T) and MJ14 appeared indistinguishable and to be most closely related to members of the genus Rhodanobacter in the family Xanthomonadaceae of the Gammaproteobacteria (96.4-98.8% sequence similarity). Strain MJ01(T) exhibited a relatively high level of DNA-DNA relatedness with strain MJ14 (89.3 %) but relatively low DNA-DNA relatedness values with established species in the genus Rhodanobacter (<60 %). The genomic DNA G+C contents of strains MJ01(T) and MJ14 were 65.3 and 64.8 mol%, respectively. The major respiratory quinone of both novel strains was the ubiquinone Q-8. The major fatty acids of both strains were iso-C(15 : 0), iso-C(16 : 0), iso-C(17:0) and iso-C(17 : 1)ω9c, and the polar lipid profiles of the two strains contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and minor amounts of unidentified aminophospholipids and phospholipids. Based on the phenotypic, genotypic and phylogenetic evidence, strains MJ01(T) and MJ14 represent a single novel species in the genus Rhodanobacter, for which the name Rhodanobacter caeni sp. nov. is proposed. The type strain is MJ01(T) ( = KCTC 22449(T) = JCM 16242(T)), with MJ14 ( = KCTC 22460 = JCM 16243) as a reference strain.
两株革兰氏反应阴性、可运动的细菌，分别命名为 MJ01(T)和 MJ14 菌株，从韩国大田污水处理厂的污泥中分离得到。使用多相方法确定了这两种菌株的分类地位。在基于 16S rRNA 基因序列的系统发育分析中，菌株 MJ01(T)和 MJ14 表现出无法区分的关系，与黄单胞菌科的 Rhodanobacter 属的成员最为密切相关（96.4-98.8%序列相似性）。菌株 MJ01(T)与 MJ14 表现出相对较高的 DNA-DNA 同源性（89.3%），但与 Rhodanobacter 属中已建立的种之间的 DNA-DNA 同源性值相对较低（<60%）。菌株 MJ01(T)和 MJ14 的基因组 DNA G+C 含量分别为 65.3 和 64.8 mol%。两种新菌株的主要呼吸醌均为泛醌 Q-8。两种菌株的主要脂肪酸均为 iso-C(15:0)、iso-C(16:0)、iso-C(17:0)和 iso-C(17:1)ω9c，两种菌株的极性脂谱均含有双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和少量未鉴定的胺磷脂和磷脂。基于表型、基因型和系统发育证据，菌株 MJ01(T)和 MJ14 代表 Rhodanobacter 属中的一个新种，建议将其命名为 Rhodanobacter caeni sp. nov.。模式株为 MJ01(T)（=KCTC 22449(T)=JCM 16242(T)），参考株为 MJ14（=KCTC 22460=JCM 16243）。
