van Rijn Vera M, Mooij Sofie H, Mollers Madelief, Snijders Peter J F, Speksnijder Arjen G C L, King Audrey J, de Vries Henry J C, van Eeden Arne, van der Klis Fiona R M, de Melker Hester E, van der Sande Marianne A B, van der Loeff Maarten F Schim
Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, the Netherlands.
Cluster of Infectious Diseases, Public Health Service of Amsterdam, Amsterdam, the Netherlands; Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
PLoS One. 2014 Mar 20;9(3):e92208. doi: 10.1371/journal.pone.0092208. eCollection 2014.
The effects of single or multiple concordant HPV infections at various anatomical sites on type-specific HPV seropositivity are currently unknown. In this cross-sectional study we assessed whether high-risk HPV infections at various anatomical sites (i.e., anal canal, penile shaft, and oral cavity), as well as concordant infections at multiple anatomical sites, were associated with type-specific seropositivity in HIV-positive and HIV-negative MSM. MSM aged ≥ 18 years were recruited in Amsterdam, the Netherlands (2010-2011). Baseline anal, penile, and oral samples were analyzed for HPV DNA and genotyped using a highly sensitive PCR and reverse line blot assay. Virus-like particle (VLP) based multiplex immunoassay was used to asses HPV-specific serum antibodies against L1 VLPs. The associations between HPV infections and type-specific seropositivity of seven high-risk HPV types (7-hrHPV: types 16, 18, 31, 33, 45, 52, 58) were estimated using logistic regression analyses with generalized estimating equations. We found that 86% of 306 HIV-positive MSM and 62% of 441 HIV-negative MSM were seropositive for at least one 7-hrHPV type. 69% of HIV-positive and 41% of HIV-negative MSM were infected with at least one 7-hrHPV type at the anus, penis, or oral cavity. In multivariable analyses, 7-hrHPV seropositivity was associated with type-specific anal (and not penile) 7-hrHPV infection, and did not significantly increase with a higher number of infected anatomical sites. Oral 7-hrHPV infection showed a positive, albeit non-significant, association with seropositivity. In conclusion, seropositivity among MSM appears to be largely associated with anal HPV infection, irrespective of additionally infected anatomical sites.
目前尚不清楚不同解剖部位的单一或多重一致性人乳头瘤病毒（HPV）感染对特定类型HPV血清阳性的影响。在这项横断面研究中，我们评估了不同解剖部位（即肛管、阴茎体和口腔）的高危HPV感染以及多个解剖部位的一致性感染是否与HIV阳性和HIV阴性男男性行为者（MSM）的特定类型血清阳性有关。2010 - 2011年在荷兰阿姆斯特丹招募了年龄≥18岁的MSM。对基线时的肛门、阴茎和口腔样本进行HPV DNA分析，并使用高灵敏度PCR和反向线印迹分析进行基因分型。基于病毒样颗粒（VLP）的多重免疫测定用于评估针对L1 VLP的HPV特异性血清抗体。使用广义估计方程的逻辑回归分析估计HPV感染与七种高危HPV类型（7种高危HPV：16、18、31、33、45、52、58型）的特定类型血清阳性之间的关联。我们发现，306名HIV阳性MSM中的86%和441名HIV阴性MSM中的62%至少对一种7种高危HPV类型呈血清阳性。69%的HIV阳性和41%的HIV阴性MSM在肛门、阴茎或口腔感染了至少一种7种高危HPV类型。在多变量分析中，7种高危HPV血清阳性与特定类型的肛门（而非阴茎）7种高危HPV感染相关，并且不会随着受感染解剖部位数量的增加而显著增加。口腔7种高危HPV感染与血清阳性呈正相关，尽管不显著。总之，MSM中的血清阳性似乎在很大程度上与肛门HPV感染有关，而与其他受感染的解剖部位无关。
