Jarvis E D, Widom R L, LaFauci G, Setoguchi Y, Richter I R, Rudner R
Department of Biological Sciences, Hunter College, City University of New York 10021.
Genetics. 1988 Nov;120(3):625-35. doi: 10.1093/genetics/120.3.625.
Integrative mapping with vectors containing ribosomal DNA sequences were used to complete the mapping of the 10 rRNA gene sets in the endospore forming bacterium Bacillus subtilis. Southern hybridizations allowed the assignment of nine operons to distinct BclI restriction fragments and their genetic locus identified by transductional crosses. Nine of the ten rRNA gene sets are located between 0 and 70 degrees on the genomic map. In the region surrounding cysA14, two sets of closely spaced tandem clusters are present. The first (rrnJ and rrnW) is located between purA16 and cysA14 closely linked to the latter; the second (rrnI, rrnH and rrnG) previously mapped within this area is located between attSPO2 and glpT6. The operons at or near the origin of replication (rrnO,rrnA and rrnJ,rrnW) represent "hot spots" of plasmid insertion.
利用含有核糖体DNA序列的载体进行整合作图，完成了芽孢杆菌属枯草芽孢杆菌中10个rRNA基因集的作图。Southern杂交将9个操纵子定位到不同的BclI限制片段上，并通过转导杂交确定了它们的遗传位点。10个rRNA基因集中的9个位于基因组图谱上0至70度之间。在cysA14周围区域，存在两组紧密间隔的串联簇。第一组（rrnJ和rrnW）位于purA16和cysA14之间，与后者紧密相连；先前定位在该区域内的第二组（rrnI、rrnH和rrnG）位于attSPO2和glpT6之间。位于复制起点或其附近的操纵子（rrnO、rrnA和rrnJ、rrnW）代表质粒插入的“热点”。
