Widom R L, Jarvis E D, LaFauci G, Rudner R
Department of Biological Sciences, Hunter College of the City University of New York, New York 10021.
J Bacteriol. 1988 Feb;170(2):605-10. doi: 10.1128/jb.170.2.605-610.1988.
Many laboratory strains of Bacillus subtilis contain 9 rather than 10 rRNA operons due to deletions occurring within the rrnJ-rrnW or rrnI-rrnH-rrnG gene cluster. These operons are members of two sets of closely spaced clusters located in the cysA-aroI region. Analysis of rescued DNA from integrants with insertions into rrnG and rrnH indicated that these tandemly arranged operons allowed frequent deletions of an rrn operon equivalent. These events may arise spontaneously by intrachromosomal recombination or by simultaneous double crossovers with a multimeric integrative plasmid.
由于rrnJ-rrnW或rrnI-rrnH-rrnG基因簇内发生缺失，许多枯草芽孢杆菌实验室菌株含有9个而非10个rRNA操纵子。这些操纵子是位于cysA-aroI区域的两组紧密间隔簇的成员。对插入rrnG和rrnH的整合体中拯救DNA的分析表明，这些串联排列的操纵子允许频繁缺失一个等效的rrn操纵子。这些事件可能通过染色体内重组或与多聚体整合质粒同时发生双交换而自发产生。
