Mikhaylova V T, Markov D V
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
J Histochem Cytochem. 1994 Dec;42(12):1643-9. doi: 10.1177/42.12.7983365.
Batches of osmium-ammine (OA) complex vary considerably in staining properties when used in a Feulgen-like reaction for selective staining of DNA-containing structures. An alternative procedure for preparation of Schiff-like reagent from OA complex is described. It is based on generation of H2SO3, respectively SO2, within the OA solution and ensures more favorable conditions for production of a Schiff-like stain than bubbling with SO2 does. The method is reproducible and yields high staining intensity. Data obtained suggest that the ability of OA complex to produce Feulgen-like staining is strongly influenced by variations in its chemical composition. Their unfavorable effect can be overcome by selecting suitable conditions for preparation of a Schiff-like reagent. Conditions for obtaining specific and sensitive Feulgen-like staining are determined.
在用于对含DNA结构进行选择性染色的类福尔根反应中，锇胺（OA）复合物批次的染色特性差异很大。本文描述了一种从OA复合物制备类席夫试剂的替代方法。该方法基于在OA溶液中分别生成H2SO3或SO2，并确保比用SO2鼓泡更有利于产生类席夫染色的条件。该方法具有可重复性，且染色强度高。所得数据表明，OA复合物产生类福尔根染色的能力受到其化学组成变化的强烈影响。通过选择制备类席夫试剂的合适条件，可以克服其不利影响。确定了获得特异性和灵敏类福尔根染色的条件。
