大蒜素对人胃癌细胞系SGC-7901的抑制作用及其机制研究。
Study on the inhibitory effect of allicin on human gastric cancer cell line SGC-7901 and its mechanism.
作者信息
Tao Mei, Gao Linghan, Pan Jie, Wang Xiaoye
机构信息
Digestive System Department, the ninth Hospital of xi'an, 710054, China.
出版信息
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):176-9. doi: 10.4314/ajtcam.v11i1.28. eCollection 2014.
BACKGROUND
Allicin is the main active constituent of Allium sativum L., which is characterized by broad antibacterial spectrum (MarkosN et al., 2008; Chen et al., 2008); it also has apparent inhibitory effects on a variety of tumors. The Objective of the paper is to study the inhibitory effect of allicin on human gastric cancer cell line SGC-7901.
MATERIALS AND METHODS
MTT assay and flow cytometry technique were applied to determine the inhibition rate of allicin on human gastric cancer cell line SGC-7901. The results shows that different concentrations of allicin apparently inhibited the gastric cancer SGC7901 cells, cell growth inhibition rates in the experimental groups showed an upward trend with increased allicin concentration, which were concentration-dependent.
RESULTS
Flow cytometry results found that the cell cycle was arrested in the G2/M phase. Allicin has an apparent inhibitory effect on proliferation of gastric cancer cells, and can induce their apoptosis.
CONCLUSION
Compared with other chemotherapeutic drugs, allicin's anti-tumor effect is better; and toxic and side effects are relatively small.
背景
大蒜素是大蒜的主要活性成分，具有抗菌谱广的特点（MarkosN等人，2008年；Chen等人，2008年）；它对多种肿瘤也有明显的抑制作用。本文的目的是研究大蒜素对人胃癌细胞系SGC - 7901的抑制作用。
材料与方法
采用MTT法和流式细胞术测定大蒜素对人胃癌细胞系SGC - 7901的抑制率。结果表明，不同浓度的大蒜素对胃癌SGC7901细胞有明显抑制作用，实验组细胞生长抑制率随大蒜素浓度增加呈上升趋势，具有浓度依赖性。
结果
流式细胞术结果发现细胞周期阻滞于G2/M期。大蒜素对胃癌细胞增殖有明显抑制作用，并能诱导其凋亡。
结论
与其他化疗药物相比，大蒜素的抗肿瘤效果较好，且毒副作用相对较小。
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