Papa F, Laszlo A, David H L
Service de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris.
Ann Inst Pasteur Microbiol. 1988 Sep-Oct;139(5):535-45. doi: 10.1016/0769-2609(88)90153-6.
The specificity of Mycobacterium tuberculosis anti-PGL-Tb1 antiserum prepared in rabbits was evaluated by an enzyme-linked immunosorbent assay. It was found that the antiserum immunoreacted with its homologous antigen but not with purified phenolic glycolipids from M. bovis BCG (mycoside B), M. kansasii (mycoside A), M. leprae (PGL-I) or M. marinum (mycoside G). The analysis of chloroform/methanol extracts from 15 strains of M. tuberculosis showed that all formed the antigen except the type strain (strain H37Rv). Chloroform/methanol extracts of M. bovis BCG, M. xenopi, M. flavescens and M. fallax cross-reacted with the immune serum. However, chloroform/methanol extracts of representative strains of 18 other mycobacterial species did not react, therefore indicating that these did not contain the antigen.
通过酶联免疫吸附测定法评估了在兔体内制备的结核分枝杆菌抗PGL-Tb1抗血清的特异性。结果发现，该抗血清与其同源抗原发生免疫反应，但不与来自卡介苗（霉菌糖苷B）、堪萨斯分枝杆菌（霉菌糖苷A）、麻风分枝杆菌（PGL-I）或海分枝杆菌（霉菌糖苷G）的纯化酚糖脂发生反应。对15株结核分枝杆菌的氯仿/甲醇提取物分析表明，除标准菌株（H37Rv菌株）外，所有菌株均形成抗原。卡介苗、蟾分枝杆菌、微黄分枝杆菌和衰败分枝杆菌的氯仿/甲醇提取物与免疫血清发生交叉反应。然而，其他18种分枝杆菌代表性菌株的氯仿/甲醇提取物未发生反应，因此表明这些菌株不含该抗原。
