芦丁增强胰岛素受体激酶，以促进胰岛素依赖性葡萄糖转运蛋白4易位。
Rutin potentiates insulin receptor kinase to enhance insulin-dependent glucose transporter 4 translocation.
作者信息
Hsu Chia-Yu, Shih Hung-Yuan, Chia Yi-Chen, Lee Chia-Hung, Ashida Hitoshi, Lai Yiu-Kay, Weng Ching-Feng
机构信息
Institute of Biotechnology, National Dong-Hwa University, Hualien, 97401, Taiwan; Institute of Biotechnology & Department of Life Science, National Tsing Hua University, Hsinchu, 30013, Taiwan.
出版信息
Mol Nutr Food Res. 2014 Jun;58(6):1168-76. doi: 10.1002/mnfr.201300691. Epub 2014 Feb 24.
SCOPE
We investigated whether rutin, a flavonoid isolated from Toona sinensis Roem, has the ability to enhance insulin-dependent receptor kinase (IRK) activity and glucose transporter 4 (GLUT4) translocation in differentiated myotubes. We also tested the effects of rutin treatment in insulin-resistant mice using an oral glucose tolerance test (OGTT).
METHODS AND RESULTS
Rutin potentiated insulin receptor kinase (IRK) phosphorylation when IRK autophosphorylation was triggered by insulin in differentiated myotubes. Co-treatment of cells with rutin and insulin attenuated S961-mediated inhibition of insulin-dependent GLUT4 translocation. In S961-treated C57BL/6 mice, an in vivo model of insulin resistance and type 2 diabetes, rutin treatment showed a normoglycemic effect in the OGTT.
CONCLUSION
This study shows evidence that rutin may serve as a potential agent for glycemic control through enhancement of IRK activity, thereby inducing the insulin signaling pathway causing increased GLUT4 translocation and increased glucose uptake.
范围
我们研究了从香椿中分离出的类黄酮芦丁是否具有增强分化肌管中胰岛素依赖性受体激酶（IRK）活性和葡萄糖转运蛋白4（GLUT4）转位的能力。我们还使用口服葡萄糖耐量试验（OGTT）测试了芦丁处理对胰岛素抵抗小鼠的影响。
方法与结果
当胰岛素在分化肌管中触发IRK自磷酸化时，芦丁增强了胰岛素受体激酶（IRK）的磷酸化。芦丁与胰岛素共同处理细胞减弱了S961介导的对胰岛素依赖性GLUT4转位的抑制作用。在S961处理的C57BL/6小鼠（一种胰岛素抵抗和2型糖尿病的体内模型）中，芦丁处理在OGTT中显示出正常血糖作用。
结论
本研究表明，芦丁可能通过增强IRK活性作为血糖控制的潜在药物，从而诱导胰岛素信号通路，导致GLUT4转位增加和葡萄糖摄取增加。
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