O'Keefe S J, Ogden J M, Young G O, Dicker J, Girdwood A H, Marks I N
Gastro-intestinal Clinic, Groote Schuur Hospital, South Africa.
Int J Pancreatol. 1989 Feb;4(1):13-27. doi: 10.1007/BF02924144.
Earlier studies have suggested that the rate of incorporation of labeled amino acids into duodenal juice proteins during pancreatic stimulation may be used to calculate pancreatic enzyme synthesis and function. In the present study, a pulse/4 h continuous intravenous infusion of 14C labeled leucine was used to compare synthesis rates in 6 patients with chronic calcific pancreatis(CP) to 4 controls. Analysis of duodenal juice protein demonstrated a delay of approximately 1 h in the appearance of labeled proteins, followed by a linear increase in specific activity, allowing calculation of synthesis that varied between 2.6-2.8 h in controls and 6-48 h in CP. The protein in controls was representative of enzyme protein, but that of CP was not, since it was heavily contaminated with albumin (up to 50%). The results indicate that enzyme secreted during the first hour of stimulation is derived from pancreatic stores and that the synthesis rate of enzymes secreted thereafter is approximately 2.7 h in normal humans. The method was, however, unable to determine rates in patients with CP owing to heavy contamination of enzymes with exudative proteins.
早期研究表明，在胰腺受到刺激时，标记氨基酸掺入十二指肠液蛋白质的速率可用于计算胰腺酶的合成和功能。在本研究中，采用脉冲/4小时连续静脉输注14C标记的亮氨酸，以比较6例慢性钙化性胰腺炎（CP）患者与4例对照者的合成速率。十二指肠液蛋白质分析显示，标记蛋白质出现延迟约1小时，随后比活性呈线性增加，从而能够计算出对照组合成时间在2.6 - 2.8小时之间，而CP患者则在6 - 48小时之间。对照组的蛋白质代表酶蛋白，但CP患者的蛋白质并非如此，因为它被白蛋白严重污染（高达50%）。结果表明，刺激后第一小时分泌的酶来自胰腺储存，此后分泌的酶在正常人体内的合成速率约为2.7小时。然而，由于酶被渗出性蛋白质严重污染，该方法无法测定CP患者的合成速率。
