Pérez de Sevilla Müller Luis, Sargoy Allison, Rodriguez Allen R, Brecha Nicholas C
Department of Neurobiology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, California, United States of America.
Department of Neurobiology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, California, United States of America; Jules Stein Eye Institute, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, California, United States of America.
PLoS One. 2014 Mar 26;9(3):e93274. doi: 10.1371/journal.pone.0093274. eCollection 2014.
We report that the most common retinal ganglion cell type that remains after optic nerve transection is the M1 melanopsin ganglion cell. M1 ganglion cells are members of the intrinsically photosensitive retinal ganglion cell population that mediates non-image-forming vision, comprising ∼2.5% of all ganglion cells in the rat retina. In the present study, M1 ganglion cells comprised 1.7±1%, 28±14%, 55±13% and 82±8% of the surviving ganglion cells 7, 14, 21 and 60 days after optic nerve transection, respectively. Average M1 ganglion cell somal diameter and overall morphological appearance remained unchanged in non-injured and injured retinas, suggesting a lack of injury-induced degeneration. Average M1 dendritic field size increased at 7 and 60 days following optic nerve transection, while average dendritic field size remained similar in non-injured retinas and in retinas at 14 and 21 days after optic nerve transection. These findings demonstrate that M1 ganglion cells are more resistant to injury than other ganglion cell types following optic nerve injury, and provide an opportunity to develop pharmacological or genetic therapeutic approaches to mitigate ganglion cell death and save vision following optic nerve injury.
我们报告称，视神经横断后残留的最常见视网膜神经节细胞类型是M1黑视蛋白神经节细胞。M1神经节细胞是介导非成像视觉的内在光敏性视网膜神经节细胞群体的成员，占大鼠视网膜所有神经节细胞的约2.5%。在本研究中，视神经横断后7、14、21和60天，M1神经节细胞分别占存活神经节细胞的1.7±1%、28±14%、55±13%和82±8%。在未受伤和受伤的视网膜中，M1神经节细胞的平均胞体直径和整体形态外观保持不变，表明缺乏损伤诱导的退化。视神经横断后7天和60天，M1树突野平均大小增加，而在未受伤的视网膜以及视神经横断后14天和21天的视网膜中，树突野平均大小保持相似。这些发现表明，视神经损伤后，M1神经节细胞比其他类型的神经节细胞更耐损伤，并为开发药理学或基因治疗方法以减轻神经节细胞死亡和挽救视神经损伤后的视力提供了机会。
