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一种基于芘的新型高灵敏度荧光探针,用于检测铜(II)和氟化物,并可应用于活细胞。

A new pyrene based highly sensitive fluorescence probe for copper(II) and fluoride with living cell application.

作者信息

Goswami Shyamaprosad, Chakraborty Shampa, Paul Sima, Halder Sandipan, Panja Sukanya, Mukhopadhyay Subhra Kanti

机构信息

Department of Chemistry, Bengal Engineering and Science University, Shibpur, Howrah 711103, West Bengal, India.

出版信息

Org Biomol Chem. 2014 May 21;12(19):3037-44. doi: 10.1039/c4ob00067f.

DOI:10.1039/c4ob00067f
PMID:24671378
Abstract

A new pyrene based fluorescence probe has been synthesized for fluorogenic detection of Cu(2+) in acetonitrile-aqueous media (7 : 3 CH3CN-HEPES buffer, v/v, at pH 7.5) with bioimaging in both prokaryotic (Candida albicans cells) and eukaryotic (Tecoma stans pollen cells) living cells. The anion recognition properties of the sensor have also been studied in acetonitrile by fluorescence methods which show remarkable sensitivity toward fluoride over other anions examined.

摘要

一种基于芘的新型荧光探针已被合成,用于在乙腈 - 水介质(7 : 3 CH3CN - HEPES缓冲液,v/v,pH 7.5)中对Cu(2+)进行荧光检测,并在原核生物(白色念珠菌细胞)和真核生物(黄钟花花粉细胞)活细胞中进行生物成像。还通过荧光方法在乙腈中研究了该传感器的阴离子识别特性,结果表明其对氟化物的敏感性显著高于其他检测的阴离子。

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