Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zijingang Campus, Zhejiang University, No. 866 Yuhangtang Rd, Hangzhou, 310058, China,
Environ Sci Pollut Res Int. 2014;21(13):8124-32. doi: 10.1007/s11356-014-2747-5. Epub 2014 Mar 27.
Elsholtzia splendens is a copper-tolerant plant species which grows on copper deposits in China. The generation of a valuable E. splendens biomass on specific contaminated sites has become one of the promising phytotechnologies. The simultaneous separations of apigenin, luteolin, and rosmarinic acid yielded in the ethyl acetate extracts of the flowering aerial parts was achieved by the use of a macroporous resin, polyamide, and silicagel columns during chromatography. Chemical identification confirmed the structures based on the spectra of FTIR, NMR, and HPLC/ESI-MS. The isolated compounds of purity above 98.3% were evaluated for their in vitro cytotoxic activities against human cancer cell lines including A549 (non-small lung), A431 (skin), and Bcap37 (breast). Among these compounds, luteolin and apigenin presented the best cytotoxic activities against A549, A431, and Bcap37 cells and, therefore, both could be the valuable products for the post-harvest processing of E. splendens biomass.
海州香薷是一种耐铜植物，在中国的铜矿上生长。在特定的污染场地，通过利用大孔树脂、聚酰胺和硅胶柱层析从开花地上部分的乙酸乙酯提取物中同时分离芹菜素、木犀草素和迷迭香酸，生成有价值的海州香薷生物量已成为一种很有前途的植物修复技术。化学鉴定基于 FTIR、NMR 和 HPLC/ESI-MS 的光谱确认了这些化合物的结构。对纯度高于 98.3%的分离化合物进行了体外细胞毒性活性评估，这些化合物针对包括 A549（非小细胞肺癌）、A431（皮肤）和 Bcap37（乳腺）在内的人癌细胞系。在这些化合物中，木犀草素和芹菜素对 A549、A431 和 Bcap37 细胞表现出最好的细胞毒性活性，因此它们都可能是海州香薷生物量收获后加工的有价值产品。
