Kim Dong Wook, Son Kun Ho, Chang Hyeun Wook, Bae KiHwan, Kang Sam Sik, Kim Hyun Pyo
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Arch Pharm Res. 2003 Mar;26(3):232-6. doi: 10.1007/BF02976835.
Elsholtzia splendens Nakai has been used in North-East Asia as an ingredient of folk medicines for treating cough, headache and inflammation. The present investigation was carried out to establish its in vivo anti-inflammatory activity using several animal models of inflammation and pain. The 75% ethanol extract of the aerial part of E. splendens significantly inhibited mouse croton oil-induced, as well as arachidonic acid-induced, ear edema by oral administration (44.6% inhibition of croton oil-induced edema at 400 mg/kg). This plant material also showed significant inhibitory activity against the mouse ear edema induced by multiple treatment of phorbol ester for 3 days, which is an animal model of subchronic inflammation. In addition, E. splendens exhibited significant analgesic activity against mouse acetic acid-induced writhing (50% inhibition at 400 mg/kg), while indomethacin (5 mg/kg) demonstrated 95% inhibition. E. splendens (5-100 microg/mL) significantly inhibited PGE2 production by pre-induced cyclooxygenase-2 of lipopolysaccharide-treated RAW 264.7 cells, suggesting that cyclooxygenase-2 inhibition might be one of the cellular mechanisms of anti-inflammation.
海州香薷在东北亚地区一直被用作治疗咳嗽、头痛和炎症的民间药物成分。本研究旨在利用多种炎症和疼痛动物模型确定其体内抗炎活性。海州香薷地上部分的75%乙醇提取物通过口服给药可显著抑制小鼠巴豆油诱导的以及花生四烯酸诱导的耳肿胀（400mg/kg时对巴豆油诱导的肿胀抑制率为44.6%）。该植物材料对佛波酯多次处理3天诱导的小鼠耳肿胀也显示出显著的抑制活性，这是一种亚慢性炎症动物模型。此外，海州香薷对小鼠醋酸诱导的扭体表现出显著的镇痛活性（400mg/kg时抑制率为50%），而吲哚美辛（5mg/kg）的抑制率为95%。海州香薷（5 - 100μg/mL）可显著抑制脂多糖处理的RAW 264.7细胞预先诱导的环氧化酶-2产生前列腺素E2，表明抑制环氧化酶-2可能是其抗炎的细胞机制之一。
