Hojati Mostafa, Modarres-Sanavy Seyed Ali Mohammad, Enferadi Sattar Tahmasebi, Majdi Mohammad, Ghanati Faezeh, Farzadfar Soudeh, Pazoki Alireza
Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, Jallal-Al-Ahmad Highway, Nasr Bridge, Tehran, Iran.
National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965-161, Tehran, Iran.
Environ Sci Pollut Res Int. 2017 May;24(13):12261-12272. doi: 10.1007/s11356-017-8846-3. Epub 2017 Mar 29.
Morphological and biochemical responses of feverfew plants exposed to low (5 μM) and high (35 and 70 μM) levels of Cd or Cu were investigated. Increasing metal supply notably reduced the plant biomass. Elevated Cd and Cu levels also resulted in an increase in the leaf proline content. Besides, decrease in ascorbic acid (AsA) and glutathione (GSH) contents was similar in the leaves of Cd- and Cu-treated plants, indicating altered biosynthesis of AsA and GSH under metal excess. High metal doses stimulated increase in antioxidative enzyme activities that could be related to elevated hydrogen peroxide (HO) content and subsequent lipid peroxidation. Cd was typically more accumulated in shoots and roots than Cu, leading to higher translocation factor at high Cd doses. In terms of essential oil content, it seems that Cd had an inhibitory effect during the experiment, whereas Cu was found to stimulate it only at 5 μM. Furthermore, high Cd supply enhanced the relative proportion of monoterpene hydrocarbons, while Cu increased the proportion of sesquiterpenes, especially at 5 μM. This result provides the first evidence of the response of feverfew plants to Cd or Cu by associating stress-related responses with changes in terpenoids.
研究了小白菊植株在低浓度（5 μM）和高浓度（35和70 μM）镉或铜处理下的形态和生化反应。增加金属供应显著降低了植株生物量。镉和铜浓度升高还导致叶片脯氨酸含量增加。此外，镉和铜处理植株叶片中抗坏血酸（AsA）和谷胱甘肽（GSH）含量的降低情况相似，表明在金属过量情况下AsA和GSH的生物合成发生了改变。高金属剂量刺激了抗氧化酶活性的增加，这可能与过氧化氢（HO）含量升高及随后的脂质过氧化有关。镉在地上部和根部的积累通常比铜更多，导致在高镉剂量下转运系数更高。就精油含量而言，在实验期间镉似乎具有抑制作用，而仅在5 μM时发现铜具有刺激作用。此外，高镉供应增加了单萜烃的相对比例，而铜增加了倍半萜的比例，尤其是在5 μM时。这一结果通过将与胁迫相关的反应与萜类化合物的变化联系起来，首次提供了小白菊植株对镉或铜反应的证据。
