Mohammadi Maedeh, Mohamed Abdul Rahman, Najafpour Ghasem D, Younesi Habibollah, Uzir Mohamad Hekarl
Faculty of Chemical Engineering, Babol Noushirvani University of Technology, Babol 47148, Iran.
Low Carbon Economy (LCE) Research Group, School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia.
ScientificWorldJournal. 2014 Jan 30;2014:910590. doi: 10.1155/2014/910590. eCollection 2014.
The intrinsic growth, substrate uptake, and product formation biokinetic parameters were obtained for the anaerobic bacterium, Clostridium ljungdahlii, grown on synthesis gas in various pressurized batch bioreactors. A dual-substrate growth kinetic model using Luong for CO and Monod for H2 was used to describe the growth kinetics of the bacterium on these substrates. The maximum specific growth rate (μ(max) = 0.195 h(-1)) and Monod constants for CO (K s,CO = 0.855 atm) and H2 (K(s,H2) = 0.412 atm) were obtained. This model also accommodated the CO inhibitory effects on cell growth at high CO partial pressures, where no growth was apparent at high dissolved CO tensions (P(CO)(∗) > 0.743 atm). The Volterra model, Andrews, and modified Gompertz were, respectively, adopted to describe the cell growth, substrate uptake rate, and product formation. The maximum specific CO uptake rate (q(max) = 34.364 mmol/g cell/h), CO inhibition constant (K(I) = 0.601 atm), and maximum rate of ethanol (R(max) = 0.172 mmol/L/h at P(CO) = 0.598 atm) and acetate (R(max) = 0.096 mmol/L/h at P(CO) = 0.539 atm) production were determined from the applied models.
在各种加压间歇式生物反应器中，以合成气为原料培养厌氧细菌Ljungdahlii梭菌，获得了其内在生长、底物摄取和产物形成的生物动力学参数。使用Luong方程描述CO的生长动力学，Monod方程描述H2的生长动力学，建立了双底物生长动力学模型，以描述该细菌在这些底物上的生长动力学。获得了最大比生长速率（μ(max)=0.195 h(-1)）以及CO（Ks,CO = 0.855 atm）和H2（K(s,H2)=0.412 atm）的Monod常数。该模型还考虑了高CO分压下CO对细胞生长的抑制作用，在高溶解CO张力（P(CO)(∗)>0.743 atm）下无明显生长。分别采用Volterra模型、Andrews模型和修正的Gompertz模型来描述细胞生长、底物摄取速率和产物形成。从应用模型中确定了最大比CO摄取速率（q(max)=34.364 mmol/g细胞/h）、CO抑制常数（K(I)=0.601 atm）以及乙醇（在P(CO)=0.598 atm时，R(max)=0.172 mmol/L/h）和乙酸盐（在P(CO)=0.539 atm时，R(max)=0.096 mmol/L/h）的最大生成速率。
