Brothman A R, Lesho L J, Somers K D, Schellhammer P F, Ladaga L E, Merchant D J
Department of Microbiology/Immunology, Eastern Virginia Medical School, Norfolk 23501.
Cancer Genet Cytogenet. 1989 Feb;37(2):241-8. doi: 10.1016/0165-4608(89)90055-1.
Primary cell cultures were established from tissue specimens obtained from patients undergoing transurethral resection of the prostate. Cytogenetic analysis of these cultures revealed a normal male chromosomal complement from one and a 45,X karyotype from another patient with benign prostatic hyperplasia. In addition, a normal male chromosomal complement was observed from a moderately differentiated prostatic carcinoma, and a grossly abnormal karyotype was observed from a poorly differentiated adenocarcinoma of the prostate. This latter specimen contained a modal chromosome number of 84 with several consistent marker chromosomes including homogeneous staining regions and double minutes, and no normal chromosomes 3, 5, 10, 15 or Y. Primary prostatic cell cultures exhibit epithelial-specific keratin intermediate filament proteins, and, in conjunction with cytogenetic analysis, provide a model for the study of human prostate cancer.
原代细胞培养是从接受经尿道前列腺切除术患者的组织标本中建立的。对这些培养物进行细胞遗传学分析，结果显示，其中一份标本具有正常男性染色体核型，另一份患有良性前列腺增生的患者标本核型为45,X。此外，在一份中度分化的前列腺癌标本中观察到正常男性染色体核型，而在一份低分化前列腺腺癌标本中观察到明显异常的核型。后一份标本的众数染色体数为84，有几条一致的标记染色体，包括均匀染色区和双微体，且没有正常的3号、5号、10号、15号染色体或Y染色体。原代前列腺细胞培养物表达上皮特异性角蛋白中间丝蛋白，结合细胞遗传学分析，为研究人类前列腺癌提供了一个模型。
