Brothman A R, Lesho L J, Somers K D, Wright G L, Merchant D J
Department of Microbiology/Immunology, Eastern Virginia Medical School, Norfolk 23501.
Int J Cancer. 1989 Nov 15;44(5):898-903. doi: 10.1002/ijc.2910440525.
A new cell line, PPC-I, has been established from a specimen obtained from a patient with a poorly differentiated adenocarcinoma of the prostate. This is the first line of its type derived from a primary prostatic tumor site. PPC-I cells have become immortalized in culture, exhibit transformation parameters including relaxed growth factor requirements and anchorage-independent growth, and are highly tumorigenic in nude mice. Cytogenetic studies by G-banding revealed a grossly abnormal karyotype, with a modal chromosome number of 84, multiple marker chromosomes including both homogeneously staining regions and double minutes and clonal loss of chromosomes 3, 5, 10, 15 and Y.
一种新的细胞系PPC-I已从一名前列腺低分化腺癌患者的标本中建立。这是首个源自原发性前列腺肿瘤部位的此类细胞系。PPC-I细胞在培养中已永生化，表现出包括对生长因子需求降低和不依赖贴壁生长在内的转化特征，并且在裸鼠中具有高度致瘤性。通过G显带进行的细胞遗传学研究显示出严重异常的核型，众数染色体数为84，有多个标记染色体，包括均匀染色区和双微体，以及3号、5号、10号、15号染色体和Y染色体的克隆性缺失。
