儿童肥胖的呼吸印记：肥胖儿童与瘦对照组相比挥发性有机化合物的变化
Breathprints of childhood obesity: changes in volatile organic compounds in obese children compared with lean controls.
作者信息
Alkhouri N, Eng K, Cikach F, Patel N, Yan C, Brindle A, Rome E, Hanouneh I, Grove D, Lopez R, Hazen S L, Dweik Raed A
机构信息
Department of Pediatric Gastroenterology and Hepatology, Cleveland Clinic, Cleveland, OH, USA; Digestive Disease Institute, Cleveland Clinic, Cleveland, OH, USA; Center for Cardiovascular Diagnostics and Prevention, Cleveland Clinic, Cleveland, OH, USA.
出版信息
Pediatr Obes. 2015 Feb;10(1):23-9. doi: 10.1111/j.2047-6310.2014.221.x. Epub 2014 Mar 27.
OBJECTIVE
The objective of this study was to investigate changes in volatile organic compounds (VOCs) in exhaled breath in overweight/obese children compared with their lean counterparts.
STUDY DESIGN
Single exhaled breath was collected and analyzed per protocol using selective ion flow tube mass spectrometry (SIFT-MS).
RESULTS
Sixty overweight/obese children and 55 lean controls were included. Compared with the lean group, the obese group was significantly older (14.1 ± 2.8 vs. 12.1 ± 3.0 years), taller (164.8 ± 10.9 vs. 153.3 ± 17.1 cm) and more likely to be Caucasian (60% vs. 35.2%); P < 0.05 for all. A comparison of the SIFT-MS results of the obese group with the lean group revealed differences in concentration of more than 50 compounds. A panel of four VOCs can identify the presence of overweight/obesity with excellent accuracy. Further analysis revealed that breath isoprene, 1-decene, 1-octene, ammonia and hydrogen sulfide were significantly higher in the obese group compared with the lean group (P value < 0.01 for all).
CONCLUSION
Obese children have a unique pattern of exhaled VOCs. Changes in VOCs observed in this study may help to gain insight into pathophysiological processes and pathways leading to the development of childhood obesity.
目的
本研究的目的是调查超重/肥胖儿童与偏瘦儿童相比，呼出气体中挥发性有机化合物（VOCs）的变化情况。
研究设计
按照方案，使用选择性离子流管质谱法（SIFT-MS）收集并分析单次呼出的气体。
结果
纳入了60名超重/肥胖儿童和55名偏瘦对照儿童。与偏瘦组相比，肥胖组年龄显著更大（14.1±2.8岁 vs. 12.1±3.0岁），身高更高（164.8±10.9厘米 vs. 153.3±17.1厘米），且更可能是白种人（60% vs. 35.2%）；所有指标P<0.05。将肥胖组与偏瘦组的SIFT-MS结果进行比较，发现50多种化合物的浓度存在差异。一组四种VOCs能够以极高的准确性识别超重/肥胖的存在。进一步分析显示，肥胖组呼出的异戊二烯、1-癸烯、1-辛烯、氨和硫化氢显著高于偏瘦组（所有P值<0.01）。
结论
肥胖儿童呼出的VOCs具有独特模式。本研究中观察到的VOCs变化可能有助于深入了解导致儿童肥胖发生的病理生理过程和途径。
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