几种部落群体作为食物消费的酸浆果实的药用特性：体外抗氧化、抗癌和抗 HIV 视角。
Medicinal attributes of Solanum xanthocarpum fruit consumed by several tribal communities as food: an in vitro antioxidant, anticancer and anti HIV perspective.
机构信息
Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
出版信息
BMC Complement Altern Med. 2014 Mar 28;14:112. doi: 10.1186/1472-6882-14-112.
BACKGROUND
Solanum xanthocarpum (Solanaceae) has been used for treatment of many infectious and degenerative diseases in traditional medicine. Present study reports the medicinal efficacy of S. xanthocarpum fruit as antioxidant, anticancer and anti HIV agents.
METHODS
Extracts were prepared using Soxhlet apparatus and partially characterized by thin layer chromatography (TLC). Total flavonoid content was determined spectrophotometrically. Reducing power, DPPH radical scavenging activity and lipid peroxidation inhibition assays were used for measurement of antioxidant potential. Cytotoxic (SRB assay) and anti-HIV RT inhibition (RT assay kit, Roche) activities were determined using ELISA.
RESULTS
TLC revealed the diversity of phytoconstituents in various sequential extracts of S. xanthocarpum fruit. Total flavonoid contents in extracts ranged between 10.22-162.49 μg quercetin equivalent/mg. Spectroscopic scanning of water soluble phenolics showed maximum absorbance at 250 and 280 nm. Polar extracts displayed potent radical scavenging activity (>80%). Several sub-fractions (spots) of extracts separated on TLC plates also exhibited powerful radical scavenging activity. Considerable reducing power was observed in extracts. Hexane fraction provided 55% lipoprotection in rat kidney homogenate. Non-polar extracts exhibited appreciable cytotoxic activity (70-91%) against leukemia (THP-1) and lung cancer (HOP-62) cell lines. Lower inhibitory activity was observed in extracts against HIV Reverse Transcriptase enzyme.
CONCLUSION
The study demonstrated considerable antioxidant and anticancer activities in S. xanthocarpum fruit.
背景
茄科植物黄果茄（Solanum xanthocarpum）在传统医学中被用于治疗多种感染性和退行性疾病。本研究报告了黄果茄果实作为抗氧化剂、抗癌和抗 HIV 药物的药用功效。
方法
使用索氏提取器制备提取物，并通过薄层色谱（TLC）进行部分表征。通过分光光度法测定总黄酮含量。使用 DPPH 自由基清除活性和脂质过氧化抑制试验测定抗氧化能力。采用 SRB 试验（SRB assay）和抗 HIV RT 抑制（RT 试验试剂盒，罗氏）测定细胞毒性（SRB assay）和抗 HIV RT 抑制（RT 试验试剂盒，罗氏）活性。
结果
TLC 显示了黄果茄果实不同连续提取物中植物成分的多样性。提取物中的总黄酮含量在 10.22-162.49μg 槲皮素当量/mg 之间。水溶性酚类物质的光谱扫描显示最大吸收在 250nm 和 280nm。TLC 板上分离的极性提取物显示出强大的自由基清除活性（>80%）。几个亚馏分（斑点）也表现出强大的自由基清除活性。提取物中观察到相当大的还原能力。己烷部分在大鼠肾匀浆中提供了 55%的脂蛋白保护。非极性提取物对白血病（THP-1）和肺癌（HOP-62）细胞系表现出明显的细胞毒性（70-91%）。在提取物中观察到对 HIV 逆转录酶的抑制活性较低。
结论
本研究表明黄果茄果实具有相当的抗氧化和抗癌活性。
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