Albrecht M A, DeLuca N A, Byrn R A, Schaffer P A, Hammer S M
Department of Medicine, New England Deaconess Hospital, Boston, Massachusetts.
J Virol. 1989 May;63(5):1861-8. doi: 10.1128/JVI.63.5.1861-1868.1989.
The interaction of human immunodeficiency virus (HIV) and herpes simplex virus (HSV) was investigated in an acute whole-virus coinfection system. CD4+ lymphoid CEM cells were infected with HIV-1 and, 24 h later, superinfected with HSV-1 (strain KOS) or HSV mutants possessing defined deletions in genes specifying the immediate-early transcriptional regulatory proteins ICP0, ICP4, or ICP27. Marked potentiation of HIV replication was demonstrated with the KOS strain, the ICP0 mutant, and the ICP27 mutant, but not with the ICP4 mutant, indicating that ICP4 is essential and ICP0 and ICP27 are nonessential for this effect. These studies demonstrate that HSV can be a potent stimulator of HIV replication and gene expression in coinfected CD4+ cells through the activity of the HSV regulatory protein ICP4.
在急性全病毒共感染系统中研究了人类免疫缺陷病毒（HIV）与单纯疱疹病毒（HSV）的相互作用。用HIV-1感染CD4 +淋巴细胞CEM细胞，24小时后，再用HSV-1（KOS株）或在指定立即早期转录调节蛋白ICP0、ICP4或ICP27的基因中具有明确缺失的HSV突变体进行超感染。KOS株、ICP0突变体和ICP27突变体均显示出对HIV复制的显著增强作用，但ICP4突变体则没有，这表明ICP4对于这种作用是必不可少的，而ICP0和ICP27则不是必需的。这些研究表明，通过HSV调节蛋白ICP4的活性，HSV可以成为共感染的CD4 +细胞中HIV复制和基因表达的有效刺激物。
