Poskin A, Martinelle L, Mostin L, Van Campe W, Dal Pozzo F, Saegerman C, Cay A B, De Regge N
Operational Directorate Viral Diseases, CODA-CERVA, Groeselenberg 99, B-1180 Brussels, Belgium; Faculty of Veterinary Medicine, University of Liège, Department of Infectious and Parasitic Diseases, Research Unit of Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Boulevard de Colonster, 20, B42, B-4000 Liège, Belgium.
Faculty of Veterinary Medicine, University of Liège, Department of Infectious and Parasitic Diseases, Research Unit of Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Boulevard de Colonster, 20, B42, B-4000 Liège, Belgium.
Vet J. 2014 Sep;201(3):419-22. doi: 10.1016/j.tvjl.2014.05.031. Epub 2014 May 27.
Schmallenberg virus (SBV) is an orthobunyavirus affecting European domestic ruminants. In this study, the dose-dependent effect of experimental infection of sheep with SBV was evaluated. Four groups of three ewes were each inoculated subcutaneously with 1 mL of successive 10-fold dilutions of an SBV infectious serum. The ewes were monitored for 10 days, but no clinical signs were observed. The number of productively infected animals within each group, as evidenced by viraemia, seroconversion and viral RNA in the organs, depended on the inoculated dose, indicating that a critical dose has to be administered to obtain a homogeneous response in infected animals under experimental conditions. In the productively infected animals, no statistical differences between the different inoculation doses were found in the duration or quantity of viral RNA circulating in blood, nor in the amount of viral RNA present in virus positive lymphoid organs.
施马伦贝格病毒（SBV）是一种影响欧洲家养反刍动物的正布尼亚病毒。在本研究中，评估了绵羊经实验感染SBV的剂量依赖性效应。将四组，每组三只母羊，分别皮下接种1 mL连续10倍稀释的SBV感染性血清。对母羊监测10天，但未观察到临床症状。每组中发生有效感染的动物数量，通过病毒血症、血清转化和器官中的病毒RNA得以证实，这取决于接种剂量，表明在实验条件下，必须给予临界剂量才能在感染动物中获得均匀一致的反应。在发生有效感染的动物中，不同接种剂量之间在血液中循环的病毒RNA的持续时间或数量，以及病毒阳性淋巴器官中存在的病毒RNA量方面，均未发现统计学差异。
