Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China; The Key Laboratory of Energy Conservation and Waste Treatment of Agricultural Structures, Ministry of Agriculture, Beijing 100081, China.
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China; The Key Laboratory of Energy Conservation and Waste Treatment of Agricultural Structures, Ministry of Agriculture, Beijing 100081, China.
J Biosci Bioeng. 2014 Sep;118(3):277-83. doi: 10.1016/j.jbiosc.2014.02.017. Epub 2014 Mar 26.
A membrane bioreactor (MBR) was developed for the treatment of anaerobically digested swine wastewater and to investigate the effect of ammonium nitrogen concentration on biological nitrogen removal and ammonia-oxidizing bacteria (AOB) community structures. The MBR achieved a high NH4(+)-N removal efficiency of 0.08 kgNMLSS(-1)d(-1) and removed 95% of the influent NH4(+)-N. The TN removal rate was highest of 82.62% at COD/TN and BOD5/TN ratios of 8.76 ± 0.30 and 3.02 ± 0.09, respectively. With the decrease in ammonium nitrogen concentrations, the diversity of the AOB community declined and showed a simple pattern of DGGE. However, the AOB population size remained high, with abundance of 10(7)-10(9) copies mL(-1). With the decrease of ammonium nitrogen concentrations, Nitrosomonas eutropha gradually disappeared, whereas Nitrosomonas sp. OZK11 showed constant adaptability to survive during each treatment stage. The selective effect of ammonium concentration on AOB species could be due to the affinity for NH4(+)-N. In this study, the changes of ammonium nitrogen concentrations in digested swine wastewater were found to have selective effects on the composition of AOB community, and biological nitrogen removal was improved by optimising the influencing parameters.
膜生物反应器（MBR）用于处理厌氧消化猪废水，并研究铵氮浓度对生物脱氮和氨氧化菌（AOB）群落结构的影响。MBR 实现了 0.08kgNMLSS(-1)d(-1)的高 NH4(+)-N 去除效率，去除了进水 NH4(+)-N 的 95%。在 COD/TN 和 BOD5/TN 比分别为 8.76±0.30 和 3.02±0.09 时，TN 去除率最高为 82.62%。随着铵氮浓度的降低，AOB 群落的多样性下降，DGGE 图谱呈现简单模式。然而，AOB 种群数量仍然很高，丰度为 10(7)-10(9)拷贝 mL(-1)。随着铵氮浓度的降低，Nitrosomonas eutropha 逐渐消失，而 Nitrosomonas sp. OZK11 在每个处理阶段都表现出恒定的适应性以存活。铵浓度对 AOB 物种的选择作用可能是由于对 NH4(+)-N 的亲和力。在本研究中，发现消化猪废水中铵氮浓度的变化对 AOB 群落组成具有选择性影响，并通过优化影响参数提高了生物脱氮效率。
