Faculty of Pharmacy, Rue Avicenne, Monastir, Tunisia.
Rheumatol Int. 2013 Mar;33(3):665-9. doi: 10.1007/s00296-012-2431-3. Epub 2012 Apr 10.
Anti-Saccharomyces cerevisiae antibodies (ASCA) had been known to be specific for Crohn's disease but it has been found in many other autoimmune diseases like systemic lupus erythematosus (SLE). Furthermore, cross-reactive epitopes on β2-glycoprotein I (β2GPI) and Saccharomyces cerevisiae were found in SLE patients. The aims of this study were to evaluate the frequency of ASCA in patients with SLE and to compare it with that of anti-β2GPI antibodies (aβ2GPI). Sera of 116 patients with SLE were analyzed in this retrospective study. All patients fulfilled at least 4 criteria of the 1997 American College of Rheumatology updated criteria for the classification of SLE. Sera of 160 blood donors were included as normal controls. ASCA IgA and IgG and aβ2GPI antibodies were determined by enzyme-linked immunosorbent assays. The frequency of ASCA (IgG and/or IgA) was significantly higher in SLE patients than in control group (31.9 vs. 3.7 %, p < 10(-6)). ASCA IgG and ASCA IgA were more frequent in SLE patients than in control group (29.3 vs. 3.1 %, p < 10(-6) and 12.1 vs. 0.6 %, p = 10(-4), respectively). The mean level of ASCA IgG was higher than that of ASCA IgA (9.5 vs. 6.4 U/ml) but the difference was not statistically significant. The frequencies of aβ2GPI (IgG and/or IgA) and aβ2GPI IgA were significantly higher than those of ASCA (IgG and/or IgA) and ASCA IgA (54.3 vs. 31.9 %, p = 5 × 10(-4) and 50.9 vs. 12.1 %, p < 10(-6), respectively). Increased ASCA IgG was observed in patients with SLE, suggesting a role of environmental stimuli in its pathogenesis.
抗酿酒酵母抗体（ASCA）以前被认为是克罗恩病的特异性抗体，但现在已经在许多其他自身免疫性疾病中发现，如系统性红斑狼疮（SLE）。此外，在 SLE 患者中发现了β2-糖蛋白 I（β2GPI）和酿酒酵母的交叉反应表位。本研究的目的是评估 ASCA 在 SLE 患者中的频率，并将其与抗β2GPI 抗体（aβ2GPI）进行比较。在这项回顾性研究中分析了 116 例 SLE 患者的血清。所有患者均符合 1997 年美国风湿病学会修订的 SLE 分类标准中的至少 4 项标准。将 160 名献血者的血清纳入正常对照组。通过酶联免疫吸附试验测定 ASCA IgG 和 IgA 及 aβ2GPI 抗体。SLE 患者 ASCA（IgG 和/或 IgA）的频率明显高于对照组（31.9%比 3.7%，p<10(-6)）。SLE 患者的 ASCA IgG 和 ASCA IgA 比对照组更常见（29.3%比 3.1%，p<10(-6)和 12.1%比 0.6%，p=10(-4)）。ASCA IgG 的平均水平高于 ASCA IgA（9.5 比 6.4 U/ml），但差异无统计学意义。aβ2GPI（IgG 和/或 IgA）和 aβ2GPI IgA 的频率明显高于 ASCA（IgG 和/或 IgA）和 ASCA IgA（54.3%比 31.9%，p=5×10(-4)和 50.9%比 12.1%，p<10(-6)）。SLE 患者中观察到 ASCA IgG 增加，提示环境刺激物在其发病机制中起作用。
