Widmann Michael, Pleiss Jürgen, Samland Anne K
Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
Institute of Microbiology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
Comput Struct Biotechnol J. 2012 Nov 13;2:e201209016. doi: 10.5936/csbj.201209016. eCollection 2012.
In this mini-review we describe the different strategies for rational protein engineering and summarize the computational tools available. Computational tools can either be used to design focused libraries, to predict sequence-function relationships or for structure-based molecular modelling. This also includes de novo design of enzymes. Examples for protein engineering of aldolases and transaldolases are given in the second part of the mini-review.
在本综述中，我们描述了合理蛋白质工程的不同策略，并总结了现有的计算工具。计算工具可用于设计聚焦文库、预测序列-功能关系或用于基于结构的分子建模。这还包括酶的从头设计。在本综述的第二部分给出了醛缩酶和转醛缩酶蛋白质工程的实例。
