Hasenson M, Lundh B, Stege R, Carlström K, Pousette A
Department of Clinical Chemistry I, Karolinska Institutet, Huddinge University Hospital, Sweden.
Prostate. 1989;14(2):83-90. doi: 10.1002/pros.2990140202.
Because a change from hormone-sensitive to hormone-resistant carcinoma of the prostate often occurs concomitantly with genetic changes or as a result of the latter, the markers specific for prostatic tissues might also be affected. We therefore first studied the presence of prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA) in LNCaP and LNCaP-r human prostatic carcinoma cell lines. Since both markers were found in these cell lines, we proceeded to quantitate PAP and PSA in aspiration biopsies from patients with prostate tumors. The amounts of these markers were compared with cytological findings. PAP and PSA were analyzed in the biopsy material from 120 patients using commercial radioimmunoassay (RIA) kits. DNA was determined using Riedel H33258 stain. Cytological grading was performed according to the Uropathological Study Group of Prostatic Carcinoma. Significant correlations were found between PAP/DNA or PSA/DNA values and grade of differentiation of the prostate tumor. In view of earlier reports and the results presented here, the amounts of markers or the protein pattern of tumor tissue may be a useful complement to the morphological findings and for selecting optimal therapy for patients with prostatic tumors.
由于前列腺癌从激素敏感型转变为激素抵抗型通常与基因变化同时发生或由基因变化引起，前列腺组织特异性标志物也可能受到影响。因此，我们首先研究了前列腺酸性磷酸酶（PAP）和前列腺特异性抗原（PSA）在LNCaP和LNCaP-r人前列腺癌细胞系中的存在情况。由于在这些细胞系中均发现了这两种标志物，我们进而对前列腺肿瘤患者穿刺活检组织中的PAP和PSA进行定量分析。将这些标志物的含量与细胞学检查结果进行比较。使用商用放射免疫分析（RIA）试剂盒对120例患者的活检材料中的PAP和PSA进行分析。采用Riedel H33258染色法测定DNA。根据前列腺癌泌尿病理研究组的标准进行细胞学分级。发现PAP/DNA或PSA/DNA值与前列腺肿瘤的分化程度之间存在显著相关性。鉴于早期报告及本文所呈现的结果，标志物的含量或肿瘤组织的蛋白质模式可能是对形态学检查结果的有益补充，有助于为前列腺肿瘤患者选择最佳治疗方案。
