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Kinetics and inhibition of reverse transcriptase from human and simian immunodeficiency viruses.人类和猿猴免疫缺陷病毒逆转录酶的动力学及抑制作用
Antimicrob Agents Chemother. 1988 Dec;32(12):1887-90. doi: 10.1128/AAC.32.12.1887.
2
Reverse transcriptases from human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus (SIVMAC) are susceptible to inhibition by foscarnet and 3'-azido-3'-deoxythymidine triphosphate.来自1型人类免疫缺陷病毒（HIV-1）、HIV-2和猴免疫缺陷病毒（SIVMAC）的逆转录酶对膦甲酸钠和3'-叠氮-3'-脱氧胸苷三磷酸敏感，易受其抑制。
Antimicrob Agents Chemother. 1988 Nov;32(11):1733-4. doi: 10.1128/AAC.32.11.1733.
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Mechanism of inhibition of human immunodeficiency virus type 1 reverse transcriptase and human DNA polymerases alpha, beta, and gamma by the 5'-triphosphates of carbovir, 3'-azido-3'-deoxythymidine, 2',3'-dideoxyguanosine and 3'-deoxythymidine. A novel RNA template for the evaluation of antiretroviral drugs.卡波韦、3'-叠氮-3'-脱氧胸苷、2',3'-二脱氧鸟苷和3'-脱氧胸苷的5'-三磷酸酯对人免疫缺陷病毒1型逆转录酶以及人DNA聚合酶α、β和γ的抑制机制。一种用于评估抗逆转录病毒药物的新型RNA模板。
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Comparison of the effect of Carbovir, AZT, and dideoxynucleoside triphosphates on the activity of human immunodeficiency virus reverse transcriptase and selected human polymerases.卡波韦、齐多夫定和三磷酸双脱氧核苷对人类免疫缺陷病毒逆转录酶及所选人类聚合酶活性影响的比较。
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Phosphorylation of 3'-azido-2',3'-dideoxyuridine and preferential inhibition of human and simian immunodeficiency virus reverse transcriptases by its 5'-triphosphate.3'-叠氮-2',3'-双脱氧尿苷的磷酸化及其5'-三磷酸酯对人免疫缺陷病毒和猿猴免疫缺陷病毒逆转录酶的优先抑制作用。
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Enzymatic characterization of human immunodeficiency virus type 1 reverse transcriptase resistant to multiple 2',3'-dideoxynucleoside 5'-triphosphates.对多种2'，3'-双脱氧核苷5'-三磷酸具有抗性的1型人类免疫缺陷病毒逆转录酶的酶学特性
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3'-Azido-3'-deoxythymidine triphosphate as an inhibitor and substrate of purified human immunodeficiency virus reverse transcriptase.3'-叠氮-3'-脱氧胸苷三磷酸作为纯化的人类免疫缺陷病毒逆转录酶的抑制剂和底物。
Antimicrob Agents Chemother. 1987 Dec;31(12):1972-7. doi: 10.1128/AAC.31.12.1972.
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Inhibition of the reverse transcriptase from HIV by 3'-azido-3'-deoxythymidine triphosphate and its threo analogue.
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Human immunodeficiency virus type 1 reverse transcriptase. 3'-Azidodeoxythymidine 5'-triphosphate inhibition indicates two-step binding for template-primer.人类免疫缺陷病毒1型逆转录酶。3'-叠氮脱氧胸苷5'-三磷酸抑制表明模板引物的两步结合。
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Mechanistic cross-talk between DNA/RNA polymerase enzyme kinetics and nucleotide substrate availability in cells: Implications for polymerase inhibitor discovery.细胞中 DNA/RNA 聚合酶酶动力学和核苷酸底物可用性之间的机制串扰：对聚合酶抑制剂发现的影响。
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2
Phosphorylation of 3'-azido-2',3'-dideoxyuridine and preferential inhibition of human and simian immunodeficiency virus reverse transcriptases by its 5'-triphosphate.3'-叠氮-2',3'-双脱氧尿苷的磷酸化及其5'-三磷酸酯对人免疫缺陷病毒和猿猴免疫缺陷病毒逆转录酶的优先抑制作用。
Antimicrob Agents Chemother. 1989 Oct;33(10):1729-34. doi: 10.1128/AAC.33.10.1729.

本文引用的文献

1
CONVERSION OF MONO- AND OLIGODEOXYRIBONUCLEOTIDES TO 5-TRIPHOSPHATES.单脱氧核糖核苷酸和寡脱氧核糖核苷酸向5-三磷酸酯的转化。
J Am Chem Soc. 1965 Apr 20;87:1785-8. doi: 10.1021/ja01086a031.
2
Molecular basis of the antineoplastic activity of 3'-amino-3'-deoxythymidine.3'-氨基-3'-脱氧胸苷抗肿瘤活性的分子基础
Mol Pharmacol. 1984 May;25(3):441-5.
3
Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS.从旧金山艾滋病患者中分离淋巴细胞病变逆转录病毒。
Science. 1984 Aug 24;225(4664):840-2. doi: 10.1126/science.6206563.
4
Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.从艾滋病患者和艾滋病前期患者中检测、分离并持续生产细胞病变逆转录病毒（HTLV-III）。
Science. 1984 May 4;224(4648):497-500. doi: 10.1126/science.6200935.
5
Infection of chimpanzees with lymphadenopathy-associated virus.黑猩猩感染淋巴结病相关病毒。
Lancet. 1984 Dec 1;2(8414):1276-7. doi: 10.1016/s0140-6736(84)92824-1.
6
Transmission of HTLV-III infection from human plasma to chimpanzees: an animal model for AIDS.人类嗜T淋巴细胞病毒III型（HTLV-III）感染从人血浆向黑猩猩的传播：一种艾滋病动物模型
Science. 1984 Nov 2;226(4674):549-52. doi: 10.1126/science.6093251.
7
Sequence of simian immunodeficiency virus from macaque and its relationship to other human and simian retroviruses.猕猴猿猴免疫缺陷病毒序列及其与其他人类和猿猴逆转录病毒的关系。
Nature. 1987;328(6130):543-7. doi: 10.1038/328543a0.
8
Isolation of T-cell tropic HTLV-III-like retrovirus from macaques.从猕猴中分离出嗜T细胞的HTLV-III样逆转录病毒。
Science. 1985 Jun 7;228(4704):1201-4. doi: 10.1126/science.3159089.
9
Inhibitory effect of 2',3'-didehydro-2',3'-dideoxynucleosides on infectivity, cytopathic effects, and replication of human immunodeficiency virus.2',3'-二脱氢-2',3'-二脱氧核苷对人类免疫缺陷病毒的感染性、细胞病变效应及复制的抑制作用
Antimicrob Agents Chemother. 1987 Jun;31(6):907-10. doi: 10.1128/AAC.31.6.907.
10
Both 2',3'-dideoxythymidine and its 2',3'-unsaturated derivative (2',3'-dideoxythymidinene) are potent and selective inhibitors of human immunodeficiency virus replication in vitro.2',3'-双脱氧胸苷及其2',3'-不饱和衍生物（2',3'-双脱氧胸苷烯）在体外都是人类免疫缺陷病毒复制的强效和选择性抑制剂。
Biochem Biophys Res Commun. 1987 Jan 15;142(1):128-34. doi: 10.1016/0006-291x(87)90460-8.

人类和猿猴免疫缺陷病毒逆转录酶的动力学及抑制作用

Kinetics and inhibition of reverse transcriptase from human and simian immunodeficiency viruses.

作者信息

Wu J C, Chernow M, Boehme R E, Suttmann R T, McRoberts M J, Prisbe E J, Matthews T R, Marx P A, Chuang R Y, Chen M S

机构信息

Department of Antimicrobial Research, Syntex Research, Mountain View, California 94043.

出版信息

Antimicrob Agents Chemother. 1988 Dec;32(12):1887-90. doi: 10.1128/AAC.32.12.1887.

DOI:10.1128/AAC.32.12.1887
PMID:2469388
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC176038/
Abstract

Reverse transcriptase from the simian immunodeficiency virus (SIV) was found to have kinetic behavior similar to that of enzyme from the human immunodeficiency virus (HIV). Michaelis constants for the substrates TTP and dGTP and inhibition constants for the inhibitors 3'-azido-3'-deoxythymidine 5'-triphosphate, 2',3'-dideoxythymidine 5'-triphosphate, and 2'-3'-dideoxyguanosine 5'-triphosphate were obtained for SIV reverse transcriptase and were found to be similar to the corresponding values for HIV reverse transcriptase. Thus, the interaction of SIV reverse transcriptase with nucleotide analogs appears to be indistinguishable from that of the HIV enzyme, suggesting that SIV/simian acquired immunodeficiency syndrome (SAIDS) is a potentially good model of AIDS.

摘要

人们发现，猿猴免疫缺陷病毒（SIV）的逆转录酶具有与人类免疫缺陷病毒（HIV）的酶相似的动力学行为。已获得SIV逆转录酶对底物三磷酸胸苷（TTP）和三磷酸脱氧鸟苷（dGTP）的米氏常数，以及对抑制剂叠氮胸苷三磷酸、双脱氧胸苷三磷酸和双脱氧鸟苷三磷酸的抑制常数，结果发现这些常数与HIV逆转录酶的相应值相似。因此，SIV逆转录酶与核苷酸类似物的相互作用似乎与HIV酶的相互作用难以区分，这表明SIV/猿猴获得性免疫缺陷综合征（SAIDS）是艾滋病的一个潜在的良好模型。