一种针对与肿瘤坏死因子受体共同下调的细胞表面抗原的细胞杀伤性单克隆抗体（抗Fas）。
A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor.
作者信息
Yonehara S, Ishii A, Yonehara M
机构信息
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
出版信息
J Exp Med. 1989 May 1;169(5):1747-56. doi: 10.1084/jem.169.5.1747.
Abstract
We have prepared an mAb specific for a human cell surface component (termed anti-Fas mAb). Anti-Fas shows cell-killing activity that is indistinguishable from the cytolytic activity of TNF. Fas antigen was characterized by western blotting, indicating that Fas antigen is a cell surface protein with a molecular weight of 200,000, which is different from the molecular weight of TNF-R. Fas antigen, however, is co-downregulated with the TNF-R when cells sensitive to the cytolytic activity of TNF are incubated with either TNF or anti-Fas. In contrast, Fas antigen on cells insensitive to TNF is not co-downregulated with the TNF-R. We suggest that the cell-killing activity of TNF is mediated by Fas antigen associated with the TNF-R.
摘要
我们制备了一种针对人细胞表面成分的单克隆抗体（称为抗Fas单克隆抗体）。抗Fas显示出与TNF的细胞溶解活性无法区分的细胞杀伤活性。通过蛋白质印迹法对Fas抗原进行了表征，表明Fas抗原是一种分子量为200,000的细胞表面蛋白，这与TNF-R的分子量不同。然而，当对TNF的细胞溶解活性敏感的细胞与TNF或抗Fas一起孵育时，Fas抗原与TNF-R会共同下调。相比之下，对TNF不敏感的细胞上的Fas抗原不会与TNF-R共同下调。我们认为TNF的细胞杀伤活性是由与TNF-R相关的Fas抗原介导的。
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本文引用的文献
1
Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.人肿瘤坏死因子：前体结构、表达及其与淋巴毒素的同源性。
Nature. 1984;312(5996):724-9. doi: 10.1038/312724a0.
2
3
Binding of human interferon alpha to cells of different sensitivities: studies with internally radiolabeled interferon retaining full biological activity.人α干扰素与不同敏感性细胞的结合：对保留完全生物活性的内部放射性标记干扰素的研究
J Virol. 1983 Mar;45(3):1168-71. doi: 10.1128/JVI.45.3.1168-1171.1983.
4
Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro.重组人肿瘤坏死因子-α：对体外正常细胞和转化细胞增殖的影响
Science. 1985 Nov 22;230(4728):943-5. doi: 10.1126/science.3933111.
5
Human tumor necrosis factor. Production, purification, and characterization.人肿瘤坏死因子。生产、纯化及特性鉴定。
J Biol Chem. 1985 Feb 25;260(4):2345-54.
6
Molecular cloning of the complementary DNA for human tumor necrosis factor.人类肿瘤坏死因子互补DNA的分子克隆
Science. 1985 Apr 12;228(4696):149-54. doi: 10.1126/science.3856324.
7
Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors.肿瘤坏死因子的成纤维细胞生长促进活性及其与其他多肽生长因子的关系。
J Exp Med. 1986 Mar 1;163(3):632-43. doi: 10.1084/jem.163.3.632.
8
A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: ramifications for the complex physiology of TNF.一种新型的肿瘤坏死因子/恶病质素是一种细胞表面细胞毒性跨膜蛋白：对肿瘤坏死因子复杂生理学的影响。
Cell. 1988 Apr 8;53(1):45-53. doi: 10.1016/0092-8674(88)90486-2.
9
Recombinant tumor necrosis factor: its effect and its synergism with interferon-gamma on a variety of normal and transformed human cell lines.重组肿瘤坏死因子：其对多种正常及转化的人细胞系的作用及其与γ干扰素的协同作用。
Eur J Cancer Clin Oncol. 1986 Apr;22(4):419-26. doi: 10.1016/0277-5379(86)90107-0.
10
Cachectin and tumour necrosis factor as two sides of the same biological coin.恶病质素与肿瘤坏死因子是同一枚生物硬币的两面。
Nature. 1986;320(6063):584-8. doi: 10.1038/320584a0.
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