Yonehara S, Ishii A, Yonehara M
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
J Exp Med. 1989 May 1;169(5):1747-56. doi: 10.1084/jem.169.5.1747.
We have prepared an mAb specific for a human cell surface component (termed anti-Fas mAb). Anti-Fas shows cell-killing activity that is indistinguishable from the cytolytic activity of TNF. Fas antigen was characterized by western blotting, indicating that Fas antigen is a cell surface protein with a molecular weight of 200,000, which is different from the molecular weight of TNF-R. Fas antigen, however, is co-downregulated with the TNF-R when cells sensitive to the cytolytic activity of TNF are incubated with either TNF or anti-Fas. In contrast, Fas antigen on cells insensitive to TNF is not co-downregulated with the TNF-R. We suggest that the cell-killing activity of TNF is mediated by Fas antigen associated with the TNF-R.
我们制备了一种针对人细胞表面成分的单克隆抗体（称为抗Fas单克隆抗体）。抗Fas显示出与TNF的细胞溶解活性无法区分的细胞杀伤活性。通过蛋白质印迹法对Fas抗原进行了表征，表明Fas抗原是一种分子量为200,000的细胞表面蛋白，这与TNF-R的分子量不同。然而，当对TNF的细胞溶解活性敏感的细胞与TNF或抗Fas一起孵育时，Fas抗原与TNF-R会共同下调。相比之下，对TNF不敏感的细胞上的Fas抗原不会与TNF-R共同下调。我们认为TNF的细胞杀伤活性是由与TNF-R相关的Fas抗原介导的。
