Stompro B E, Hansbrough J F, Boyce S T
Department of Surgery, University of California, San Diego Medical Center 92103.
J Surg Res. 1989 May;46(5):413-21. doi: 10.1016/0022-4804(89)90153-4.
Ingrowth of fibrovascular tissue from the woundbed into collagen-based dermal substitutes and survival of cultured epithelium after transplantation may be enhanced by attachment of heparin binding growth factor 2 (HBGF2) and epidermal growth factor (EGF) to collagen. Biotinylation of collagen and the growth factors allows immobilization of HBGF2 and EGF by high affinity binding of tetravalent avidin. Biotinylated HBGF2 and EGF (B-GF) were exposed to complexes of biotinylated collagen (B-COL)-avidin (A) and detected with peroxidase-labeled avidin (AP) followed by chromagen formation on nitrocellulose paper. Binding of biotinylated HBGF2 and EGF was specific (*, P less than 0.05), proportional to the concentration of biotinylated collagen, and resistant to ionic (NaCl) displacement. Data are expressed as mean percentages of maximum binding +/- SEMs: (table; see text) Growth response of cultured human epidermal keratinocytes to HBGF2 (population doubling time, PDT = 0.70 population doublings (PD)/day) confirmed the retention of mitogenic activity after biotinylation (PDT = 0.80 PD/day). Specific binding of biotinylated HBGF2, EGF, or other biologically active molecules (antibiotics, NSAIDs) to implantable collagen may provide a mechanism for positive therapeutic modulation of wound healing, including repair of full-thickness skin wounds with cultured cell-collagen composite grafts.
通过将肝素结合生长因子2（HBGF2）和表皮生长因子（EGF）附着于胶原蛋白，可增强从创面床长入基于胶原蛋白的真皮替代物的纤维血管组织的生长以及移植后培养上皮的存活。胶原蛋白和生长因子的生物素化可通过四价抗生物素蛋白的高亲和力结合实现HBGF2和EGF的固定。将生物素化的HBGF2和EGF（B-GF）与生物素化胶原蛋白（B-COL）-抗生物素蛋白（A）的复合物接触，并用过氧化物酶标记的抗生物素蛋白（AP）进行检测，随后在硝酸纤维素纸上形成显色剂。生物素化的HBGF2和EGF的结合具有特异性（*，P小于0.05），与生物素化胶原蛋白的浓度成正比，并且对离子（NaCl）置换具有抗性。数据表示为最大结合的平均百分比±标准误：（表格；见正文）培养的人表皮角质形成细胞对HBGF2的生长反应（群体倍增时间，PDT = 0.70群体倍增数（PD）/天）证实了生物素化后促有丝分裂活性的保留（PDT = 0.80 PD/天）。生物素化的HBGF2、EGF或其他生物活性分子（抗生素、非甾体抗炎药）与可植入胶原蛋白的特异性结合可能为伤口愈合的积极治疗调节提供一种机制，包括用培养的细胞 - 胶原蛋白复合移植物修复全层皮肤伤口。
