He Fa, Wang Miao, Gao Minghuan, Zhao Min, Bai Yuhua, Zhao Chunjie
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road Shenhe District , Shenyang 110016, China.
College of Pharmacy, Harbin Medical University, Daqing 163319, China.
Molecules. 2014 Apr 2;19(4):4046-57. doi: 10.3390/molecules19044046.
Gerbera anandria (Compositae) was extracted with 75% ethanol and the residue was fractionated using light petroleum, chloroform and ethyl acetate. The constituents of the extracts were separated by column chromatography employing solvents of different polarity. Column chromatography of the light petroleum fraction resulted in the isolation of methyl hexadecanoate, while the chloroform fraction afforded xanthotoxin, 2-hydroxy-6-methylbenzoic acid, 7-hydroxy-1(3H)-isobenzofuranone, a mixture of β-sitosterol and stigmasterol, and 8-methoxysmyrindiol and the ethyl acetate fraction gave gerberinside, apigenin-7-O-β-d-glucopyranoside and quercetin. A new coumarin, 8-methoxysmyrindiol, was found. The chemical structures of the isolated compounds were established by MS and NMR (HSQC, HMBC). Free radical scavenging and cytotoxic activities of crude extracts and 8-methoxysmyrindiol were further investigated. The ethyl acetate phase exerted the strongest DPPH free radical scavenging activity in comparison to the other fractions. The coumarin 8-methoxysmyrindiol demonstrated cytotoxicity against multiple human cancer cell lines, with the highest potency in HepG2 cells.
用75%乙醇提取非洲菊（菊科），残留物依次用石油醚、氯仿和乙酸乙酯进行分离。提取物的成分通过使用不同极性溶剂的柱色谱法进行分离。石油醚部分的柱色谱分离得到十六烷酸甲酯，氯仿部分得到花椒毒素、2-羟基-6-甲基苯甲酸、7-羟基-1(3H)-异苯并呋喃酮、β-谷甾醇和豆甾醇的混合物以及8-甲氧基辛迪二醇，乙酸乙酯部分得到非洲菊苷、芹菜素-7-O-β-D-葡萄糖苷和槲皮素。发现了一种新的香豆素，8-甲氧基辛迪二醇。通过质谱和核磁共振（HSQC、HMBC）确定了分离化合物的化学结构。进一步研究了粗提物和8-甲氧基辛迪二醇的自由基清除和细胞毒性活性。与其他部分相比，乙酸乙酯相表现出最强的DPPH自由基清除活性。香豆素8-甲氧基辛迪二醇对多种人类癌细胞系具有细胞毒性，对HepG2细胞的活性最高。
