Oltulu Y M, Coskunpinar E, Ozkan G, Aynaci E, Yildiz P, Isbir T, Yaylim I
Department of Molecular Medicine, Institute of Experimental Medicine Research, Istanbul University DETAE, P.O. Box 7, 34390 Çapa/İstanbul, Turkey.
Department of Chest Diseases, Faculty of Medicine, Medipol University, Istanbul, Turkey.
Biomed Res Int. 2014;2014:530381. doi: 10.1155/2014/530381. Epub 2014 Feb 23.
Lung cancer is a complex, multifactorial disease which is the leading cause of cancer death in both men and women. NF-κB is a transcription factor which is known to affect the expression of more than 150 genes related to inflammation, lymphocyte activation, cell proliferation, differentiation, and apoptosis, as well as contributing to cell apoptosis and survival. However, NF-κBIA (IκBα) is the inhibitor of the transcription factor. The--94ins/delATTG polymorphism of the NF-κB1 gene promoter region which causes a functional effect and NF-κBIA 3'UTR A → G polymorphism has been shown to be related to various inflammatory diseases and cancer. Ninety-five NSCLC patients and 99 healthy controls were included in study. The NF-κB1-94ins/delATTG and NF-κBIA 3'UTR A → G polymorphism have been studied by using PCR-RFLP method. It was found that the NF-κB1 -94ins/delATTG DD genotype and D allele frequencies were higher in patients than healthy controls and the presence of the DD genotype has a 3.5-fold increased risk of the disease (P: 0.014). This study is the first to investigate the NF-κB1-94ins/delATTG and NF-κBIA 3'UTR A → G polymorphism together in the Turkish population. According to the results, the NF-κB1-94ins/del ATTG promoter polymorphism may have a role in lung carcinogenesis and prognosis.
肺癌是一种复杂的多因素疾病，是男性和女性癌症死亡的主要原因。核因子κB（NF-κB）是一种转录因子，已知其可影响150多个与炎症、淋巴细胞活化、细胞增殖、分化和凋亡相关的基因的表达，同时也参与细胞凋亡和存活过程。然而，NF-κB抑制因子α（NF-κBIA，即IκBα）是该转录因子的抑制剂。已证实，NF-κB1基因启动子区域的-94ins/delATTG多态性会产生功能效应，且NF-κBIA 3'非翻译区A→G多态性与多种炎症性疾病及癌症相关。本研究纳入了95例非小细胞肺癌（NSCLC）患者和99名健康对照者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对NF-κB1 -94ins/delATTG和NF-κBIA 3'UTR A→G多态性进行了研究。结果发现，患者中NF-κB1 -94ins/delATTG的DD基因型和D等位基因频率高于健康对照者，且DD基因型患者患该病的风险增加了3.5倍（P = 0.014）。本研究首次在土耳其人群中同时调查了NF-κB1-94ins/delATTG和NF-κBIA 3'UTR A→G多态性。根据研究结果，NF-κB1-94ins/del ATTG启动子多态性可能在肺癌发生和预后中发挥作用。
